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诃子提取物对角质细胞和成纤维细胞增殖的影响：一种创伤愈合的替代方法。

Effect of Extracts of Terminalia chebula on Proliferation of Keratinocytes and Fibroblasts Cells: An Alternative Approach for Wound Healing.

机构信息

Department of Nano, Medical & Polymer Materials, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsangbuk-do 712749, Republic of Korea ; YU-ECI Medical Research Center, Yeungnam University, Gyeonsanbuk-do 712749, Republic of Korea.

Department of Nano, Medical & Polymer Materials, College of Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsangbuk-do 712749, Republic of Korea.

出版信息

Evid Based Complement Alternat Med. 2014;2014:701656. doi: 10.1155/2014/701656. Epub 2014 Feb 26.

DOI:10.1155/2014/701656
PMID:24719644
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3955618/
Abstract

Terminalia chebula is one of the traditional medicines used in the treatment of many diseases. In the present work, different concentrations of various organic and aqueous extracts (solvent-free) of T. chebula were tested on fibroblast (L929) and keratinocytes cells to evaluate its biocompatible concentration by using MTT and live-dead viability/cytotoxic assay. These extracts were found to be effective in decreasing the ammonia accumulation in the media, thereby reducing its toxic effect on cells. DPPH assay further confirmed the free-radical scavenging ability of the extracts which increased with the increase in concentration of each extract. Cell proliferation/apoptosis, cytoskeletal structure, and ECM production were further evaluated by live-dead assay and phalloidin/cytokeratin staining, respectively. The cytoskeletal structure and ECM secretion of the cells treated with extracts showed higher cellular activity in comparison to control. In conclusion, we have demonstrated the effect of these extracts of T. chebula on both types of skin cells and optimized concentration in which it could be used as a bioactive component for wound healing applications by increasing cell proliferation and decreasing free-radical production without affecting the normal cellular matrix. It can also find applications in other therapeutics applications where ammonia toxicity is a limiting factor.

摘要

诃子是一种传统的药物，用于治疗许多疾病。在本工作中，用 MTT 和死活细胞活力/细胞毒性试验，对不同浓度的各种有机溶剂和水提（无溶剂）诃子提取物进行了测试，以评估其对成纤维细胞（L929）和角质细胞的生物相容性浓度。这些提取物可有效减少培养基中氨的积累，从而降低其对细胞的毒性作用。DPPH 试验进一步证实了提取物的自由基清除能力，随着每种提取物浓度的增加而增加。通过死活细胞检测和鬼笔环肽/细胞角蛋白染色，进一步评估了细胞增殖/凋亡、细胞骨架结构和细胞外基质（ECM）的产生。与对照相比，用提取物处理的细胞的细胞骨架结构和 ECM 分泌显示出更高的细胞活性。总之，我们已经证明了这些诃子提取物对两种皮肤细胞的作用，并优化了其浓度，可作为生物活性成分用于伤口愈合应用，增加细胞增殖，减少自由基生成，而不影响正常细胞基质。它还可以在其他氨毒性是限制因素的治疗应用中找到应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/091492c33704/ECAM2014-701656.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/f19e3d870474/ECAM2014-701656.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/be636962b158/ECAM2014-701656.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/6a0f0980bf15/ECAM2014-701656.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/460aafc4f93c/ECAM2014-701656.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/9984a3d70fe6/ECAM2014-701656.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/0cb0c4cd4104/ECAM2014-701656.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/250d96f58aea/ECAM2014-701656.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/6d4ee3cd692c/ECAM2014-701656.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/091492c33704/ECAM2014-701656.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/f19e3d870474/ECAM2014-701656.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/be636962b158/ECAM2014-701656.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/6a0f0980bf15/ECAM2014-701656.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/460aafc4f93c/ECAM2014-701656.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/9984a3d70fe6/ECAM2014-701656.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/0cb0c4cd4104/ECAM2014-701656.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/250d96f58aea/ECAM2014-701656.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/6d4ee3cd692c/ECAM2014-701656.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3237/3955618/091492c33704/ECAM2014-701656.009.jpg

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