Division of Cardiovascular Medicine, Department of Medicine and Health Sciences, Linköping University, Linköping, Sweden.
J Clin Lab Anal. 2010;24(4):219-23. doi: 10.1002/jcla.20389.
Examination of Exhaled Breath Condensate has been suggested to give information about inflammatory airway diseases.
The aim was to compare efficacy and variability in gain of two commercially available exhaled breath condensers, ECoScreen and RTube in an in vitro set up.
Test fluids containing myeloperoxidase (MPO) or human neutrophil lipocalin (HNL) in addition to saline and bovine serum albumin were nebulized and aerosols were transferred by a servo ventilator to either of the two condensers. Analyses of MPO, HNL, or chlorine were done by means of ELISA, RIA, or a modified adsorbed organic halogen technique (AOX), respectively.
Recoveries of HNL were higher when using ECoScreen than RTube (P<0.05). In contrast, there were no significant differences between the two condensers in recoveries of MPO or chlorine. The spread of data was wide regarding all tested compounds.
Variability in gain was large and ECoScreen was more efficacious then RTube in condensing the tested solutes of HNL, but not those of MPO or chlorine.
呼气冷凝物的检查被认为可以提供有关炎症性气道疾病的信息。
本研究旨在比较两种市售呼气冷凝物收集器（ECoScreen 和 RTube）在体外条件下的效能和变异性。
将含有髓过氧化物酶（MPO）或人中性粒细胞碱性磷酸酶（HNL）的测试液以及盐水和牛血清白蛋白雾化后，使用伺服通风机将气溶胶转移至两种冷凝物收集器中的任意一种。通过 ELISA、RIA 或改良的吸附有机卤素技术（AOX）分别检测 MPO、HNL 或氯。
使用 ECoScreen 收集 HNL 的回收率高于 RTube（P<0.05）。相比之下，两种冷凝物收集器在 MPO 或氯的回收率方面没有显著差异。所有测试化合物的数据分布都很广泛。
回收率的变异性很大，ECoScreen 在浓缩 HNL 等测试溶质方面比 RTube 更有效，但对 MPO 或氯则不然。
