Li Jian, Wang Zixu, Shi Dazhao, Chen Yaoxing
Laboratory of Veterinary Anatomy, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Toxicol Ind Health. 2010 Nov;26(10):691-700. doi: 10.1177/0748233710377771. Epub 2010 Jul 13.
Effects of sasanguasaponin (SGS), a compound from defatted seeds of Camellia oleifera, on abnormal spermatogenesis were studied in adult male mice using daily intragastric administration of 100, 200 and 400 mg/kg body weight (BW) for 6 weeks. The sperm counts decreased and the number of abnormal spermatozoa increased in mice treated with SGS at 400 mg/kg dose level. Testicular weight and seminiferous tubular area gradually decreased as dosage increased to 400 mg/kg SGS. Germ cells were rarefied and showed irregular distribution in seminiferous tubules (STs). Apoptosis was pronounced among spermatocytes and spermatids at the 400 mg/kg dose level. Antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxide capacity (T-AOC) markedly decreased and malondialdehyde (MDA) concentration increased in the testes. These results suggest that adult exposure to SGS (400 mg/kg) induces spermatogenic apoptosis through increased oxidative stress in male mice.
利用成年雄性小鼠，通过每日灌胃给予100、200和400毫克/千克体重（BW）的油茶籽脱脂种子中的一种化合物——油茶皂苷（SGS），持续6周，研究了其对异常精子发生的影响。在400毫克/千克剂量水平下用SGS处理的小鼠精子数量减少，异常精子数量增加。随着SGS剂量增加到400毫克/千克，睾丸重量和生精小管面积逐渐减小。生精小管中的生殖细胞稀少且分布不规则。在400毫克/千克剂量水平下，精母细胞和精子细胞中的凋亡明显。睾丸中的抗氧化酶，包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（T-AOC）显著降低，丙二醛（MDA）浓度升高。这些结果表明，成年雄性小鼠暴露于SGS（400毫克/千克）会通过增加氧化应激诱导生精细胞凋亡。
