Department of Medical Biochemistry, Molecular Biology Unit, Faculty of Medicine, Cairo University, Cairo, Egypt.
Andrologia. 2010 Aug;42(4):236-41. doi: 10.1111/j.1439-0272.2009.00983.x.
This work aimed to assess seminal plasma heme oxygenase (HO) enzyme activity in oligoasthenoteratozoospermia (OAT) males with varicocele. Ninety-three men were divided according to their sperm count and clinical examination into: healthy fertile controls (n = 34), OAT without varicocele (n = 37) and OAT associated with varicocele (n = 22). They were subjected to semen analysis and estimation of seminal plasma HO enzyme activity in the form of bilirubin concentration. Seminal plasma HO enzyme activity decreased significantly in OAT cases compared with controls. Seminal plasma HO in OAT cases associated with varicocele decreased significantly compared with OAT cases without varicocele and healthy controls (mean +/- SD; 109.2 +/- 29.5, 283.6 +/- 88.4, 669.5 +/- 236.1 nMol bilirubin/mg ptn/min, P < 0.001). There was positive correlation between seminal plasma HO enzyme activity and sperm concentration, per cent of motile spermatozoa, number of motile spermatozoas ml(-1) and significant negative correlation with sperm abnormal forms per cent. It is concluded that varicocele has a negative impact on seminal HO enzyme activity. Therefore, improved seminal picture after correcting varicocele repair might be related, in part, to improved HO action(s).
本研究旨在评估精索静脉曲张性少弱畸精子症（OAT）患者精浆血红素氧合酶（HO）酶活性。93 名男性根据精子计数和临床检查分为：健康生育对照组（n = 34）、无精索静脉曲张的 OAT 组（n = 37）和伴有精索静脉曲张的 OAT 组（n = 22）。他们接受精液分析和精浆 HO 酶活性（以胆红素浓度表示）的测定。与对照组相比，OAT 患者的精浆 HO 酶活性显著降低。与无精索静脉曲张的 OAT 患者和健康对照组相比，伴有精索静脉曲张的 OAT 患者的精浆 HO 活性显著降低（均值 ± SD；109.2 ± 29.5、283.6 ± 88.4、669.5 ± 236.1 nMol 胆红素/mg ptn/min，P < 0.001）。精浆 HO 酶活性与精子浓度、前向运动精子百分率、精子/ml 的运动精子数呈正相关，与精子畸形百分率呈负相关。精索静脉曲张对精浆 HO 酶活性有负面影响。因此，精索静脉曲张修复后精液质量改善可能部分与 HO 活性增强有关。
