与中国大菱鲆死亡相关的虹彩病毒（虹彩病毒科）的完整基因组序列。
Complete genome sequence of a Megalocytivirus (family Iridoviridae) associated with turbot mortality in China.
机构信息
Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Qingdao 266071, China.
出版信息
Virol J. 2010 Jul 15;7:159. doi: 10.1186/1743-422X-7-159.
BACKGROUND
Turbot reddish body iridovirus (TRBIV) causes serious systemic diseases with high mortality in the cultured turbot, Scophthalmus maximus. We here sequenced and analyzed the complete genome of TRBIV, which was identified in Shandong province, China.
RESULTS
The genome of TRBIV is a linear double-stranded DNA of 110,104 base pairs, comprising 55% G + C. Total 115 open reading frames were identified, encoding polypeptides ranging from 40 to 1168 amino acids. Amino acid sequences analysis revealed that 39 of the 115 potential gene products of TRBIV show significant homology to other iridovirus proteins. Phylogenetic analysis of conserved genes indicated that TRBIV is closely related to infectious spleen and kidney necrosis virus (ISKNV), rock bream iridovirus (RBIV), orange-spotted grouper iridovirus (OSGIV), and large yellow croaker iridovirus (LYCIV). The results indicated that TRBIV belongs to the genus Megalocytivirus (family Iridoviridae).
CONCLUSIONS
The determination of the genome of TRBIV will provide useful information for comparative study of Megalocytivirus and developing strategies to control outbreaks of TRBIV-induced disease.
背景
大菱鲆红体虹彩病毒（TRBIV）可引起养殖大菱鲆严重的全身性疾病，死亡率很高。我们在这里对中国山东省鉴定的 TRBIV 完整基因组进行了测序和分析。
结果
TRBIV 的基因组是一条线性双链 DNA，全长 110104 个碱基对，包含 55%的 G+C。共鉴定出 115 个开放阅读框，编码的多肽长度从 40 到 1168 个氨基酸不等。氨基酸序列分析显示，TRBIV 的 115 个潜在基因产物中有 39 个与其他虹彩病毒蛋白具有显著同源性。保守基因的系统发育分析表明，TRBIV 与传染性脾肾坏死病毒（ISKNV）、石斑鱼虹彩病毒（RBIV）、橙点石斑鱼虹彩病毒（OSGIV）和大黄鱼虹彩病毒（LYCIV）密切相关。结果表明，TRBIV 属于 Megalocytivirus 属（虹彩病毒科）。
结论
TRBIV 基因组的确定将为比较 Megalocytivirus 研究和制定控制 TRBIV 诱导疾病爆发的策略提供有用信息。
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