Yoo Jung Sun, Kim Hong Bae, Ogay Vyacheslav, Lee Byung-Cheon, Ahn Saeyoung, Soh Kwang-Sup
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea.
J Acupunct Meridian Stud. 2009 Jun;2(2):118-23. doi: 10.1016/S2005-2901(09)60043-1.
The present study has been designed to find a possible new route for the metastasis of cancer cells on the fascia surrounding tumor tissue using a novel technique of trypan blue staining.
Tumor tissues were grown in the skin of nude mice after sub-cutaneous inoculation with human lung cancer cells. Trypan blue was recently identified as a dye with specificity for Bonghan ducts (BHDs) and not other tissues, such as blood or lymph vessels or nerves.
We demonstrate that the trypan blue staining technique allows the first visualization of BHDs which are connected to tumor tissues.
Since BHDs are known to make up a circulatory system corresponding to acupuncture meridians or collaterals, we propose that, in addition to the currently known blood or lymph vessels, BHDs on tumor tissue fascia may be a novel pathway for metastasis.
本研究旨在利用锥虫蓝染色新技术寻找癌细胞在肿瘤组织周围筋膜上转移的可能新途径。
将人肺癌细胞皮下接种于裸鼠皮肤后培养肿瘤组织。锥虫蓝最近被鉴定为一种对凤汉管（BHDs）具有特异性的染料，而对血液、淋巴管或神经等其他组织无特异性。
我们证明锥虫蓝染色技术首次使连接肿瘤组织的BHDs可视化。
由于已知BHDs构成了与经络相对应的循环系统，我们提出，除了目前已知的血管或淋巴管外，肿瘤组织筋膜上的BHDs可能是转移的新途径。
