Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, and University of Science and Technology of China, Hefei, China.
Cell Signal. 2011 Jan;23(1):1-5. doi: 10.1016/j.cellsig.2010.07.003. Epub 2010 Jul 12.
Protein kinase and phosphatase signaling cascade, coupled with other post-translational modifications, orchestrates temporal order of various events during cell division. Among the many mitotic kinases, Polo-like kinase 1 (PLK1) as a key regulator, participates in regulating mitosis from mitotic entry to cytokinesis. The advancement in optical reporter engineering and the recent development of specific chemical probes enable us to visualize spatiotemporal gradient of kinase activity at nano-scale. One of such tools is FRET-based optic sensor that allows us to delineate the PLK1 activity in space and time. In this review, we address the inter-relationships between PLK1 and other protein kinases/phosphatases, as well as the crosstalk between PLK1 phosphorylation and ubiquitination during cell division. In particular, we discuss the molecular mechanisms and steps underlying PLK1 kinase priming, activation and turn-off during cell division.
蛋白激酶和磷酸酶信号级联反应，与其他翻译后修饰一起，协调细胞分裂过程中各种事件的时间顺序。在众多有丝分裂激酶中，Polo 样激酶 1（PLK1）作为关键调节因子，参与调节从有丝分裂进入到胞质分裂的有丝分裂。光学报告基因工程的进步和特定化学探针的最新发展使我们能够在纳米尺度上可视化激酶活性的时空梯度。这样的工具之一是基于 FRET 的光学传感器，它允许我们在空间和时间上描绘 PLK1 活性。在这篇综述中，我们讨论了 PLK1 与其他蛋白激酶/磷酸酶之间的相互关系，以及细胞分裂过程中 PLK1 磷酸化和泛素化之间的串扰。特别是，我们讨论了细胞分裂过程中 PLK1 激酶引发、激活和关闭的分子机制和步骤。
