Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Erlangen, Germany.
Clin Oral Implants Res. 2010 Jul;21(7):709-17. doi: 10.1111/j.1600-0501.2010.01914.x.
The number of diabetic patients in need of medical treatment is growing steadily. Therefore, a diabetic animal model with high degree of similarities with humans, which is suitable for the systematic evaluation of biomaterials and medical devices, is needed.
Twenty domestic pigs were used for the study. Fifteen received Streptozotocin (STZ) to induce diabetes mellitus. Internal parameters were measured and bone as well as soft tissues biopsies were taken after 0, 6 and 12 months and evaluated qualitatively and quantitatively by means of scanning electronic microscopy, light microscopy and microradiography.
The results of the clinical internal parameters, determined by the American Diabetes Association for the definition of diabetes mellitus could be fulfilled. Pathological changes of the skin vasculatures were already visible after 6 months with a significant wall thickening in the diabetic group. The bone mineralization was lower in the diabetic group after 6 months and with a significant difference after 12 months.
From the present results, it can be concluded that a STZ dosage of 90 mg/kg body weight in the domestic pig is suitable for the induction of an apparent diabetes, leading to histolopathological changes in the hard and soft tissues already after 6 months. The high degree of similarities with humans makes it an interesting diabetic animal model for biomaterial research in a compromised animal model.
需要治疗的糖尿病患者数量正在稳步增长。因此，需要一种与人类高度相似的糖尿病动物模型，该模型适用于生物材料和医疗器械的系统评估。
本研究使用了 20 只国产猪。其中 15 只接受链脲佐菌素（STZ）诱导糖尿病。在 0、6 和 12 个月后测量内部参数，并通过扫描电子显微镜、光学显微镜和微射线照相术对骨和软组织活检进行定性和定量评估。
通过美国糖尿病协会确定的糖尿病定义，临床内部参数的结果可以得到满足。6 个月后，糖尿病组的皮肤血管出现明显的病理变化，血管壁明显增厚。6 个月后，糖尿病组的骨矿化程度较低，12 个月后差异显著。
从目前的结果可以得出结论，90mg/kg 体重的 STZ 剂量可用于诱导明显的糖尿病，导致硬组织和软组织的组织病理学变化在 6 个月后就已经出现。与人类的高度相似性使它成为一种有趣的糖尿病动物模型，适用于生物材料在受损动物模型中的研究。
