Department of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, China.
J Pharm Pharmacol. 2010 Jul;62(7):915-23. doi: 10.1211/jpp.62.07.0013.
This study aimed to investigate the possible modification of the neuroprotective effect of sodium ferulate, when orally co-administered with borneol, in transient global cerebral ischaemia-induced functional, histological and cellular alterations in mice.
The bilateral common carotid artery occlusion was conducted in C57 BL/6J mice for 25 min. The mice were then subjected to a water maze test over an extended recovery period, followed by an assessment of neuronal loss in the CA1 region of the hippocampus (haematoxylin and eosin staining). The blood-brain barrier permeability (Evans blue tracing), brain oedema and oxidative stress were assayed and histological sections were also immunostained for gliofibrillar acid protein (GFAP) expression.
The ischaemia reperfused mice were associated with long-lasting spatial learning deficits in the absence of other behavioural impairments and with neurodegeneration in the hippocampal CA1 region. However, the histological injuries were significantly attenuated by oral co-administration of sodium ferulate and borneol. Furthermore, combined treatment with sodium ferulate and borneol resulted in a significant reduction in brain oedema, GFAP-positive cells, malonaldialdehyde levels and blood-brain barrier permeability, but an increase in superoxide dismutase activity.
Borneol may have benefits for the neuroprotective effect of sodium ferulate against injury induced in the brain by ischaemia/reperfusion.
本研究旨在探讨当归龙荟丸是否能增强阿魏酸钠对短暂全脑缺血诱导的功能、组织学和细胞改变的神经保护作用。
采用双侧颈总动脉闭塞法制作 C57BL/6J 小鼠短暂全脑缺血模型，缺血 25 min 后再灌注，再灌注后进行水迷宫实验，延长恢复期，然后检测海马 CA1 区神经元丢失（苏木精-伊红染色）。测定血脑屏障通透性（伊文思蓝示踪法）、脑水肿和氧化应激，并对胶质纤维酸性蛋白（GFAP）表达进行免疫组织化学染色。
缺血再灌注小鼠出现持续的空间学习障碍，无其他行为障碍，海马 CA1 区神经退行性变。然而，阿魏酸钠和当归龙荟丸联合口服可显著减轻组织学损伤。此外，阿魏酸钠和当归龙荟丸联合治疗可显著降低脑水肿、GFAP 阳性细胞数、丙二醛水平和血脑屏障通透性，同时增加超氧化物歧化酶活性。
当归龙荟丸可能增强阿魏酸钠对缺血再灌注诱导的脑损伤的神经保护作用。
