Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences de Gafsa, Cité Zarroug, 2112 Gafsa, Tunisia.
Phytomedicine. 2010 Dec 1;17(14):1120-6. doi: 10.1016/j.phymed.2010.05.001. Epub 2010 Jul 16.
Opuntia ficus indica f. inermis methanolic root extract (ORE) was investigated for phenolic and flavonoids contents, in vitro evaluated for DPPH radical scavenging activity, reducing power and in vivo tested for its gastro-protective ability against 80% ethanol induced ulcer in rats. Phytochemical test of ORE were positive for phenolic and flavonoid contents. DPPH radical scavenging activity and reducing power of ORE showed an EC(50) of 118.65±2.51 μg/ml and 300 μg/ml respectively. In vivo the pre-treatment of rats with ranitidine (50 mg/kg) and 200, 400, and 800 mg/kg doses of ORE significantly (p<0.05) reduced the 80% ethanol induced-ulcer lesion, with a rate of 82.68%, 49.21%, 83.13%, and 92.59% respectively, and prevented the depletion of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH), and inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA) in rat stomach tissues when compared with ethanol group. Also pre-treatment with ORE marked a dose-dependent attenuation of histopathology changes induced by ethanol. Phenolic and flavonoids wealth, radical scavenging activity, and reducing power, have been implicated for antiulcer property of ORE.
仙人掌果实甲醇根提取物（ORE）的酚类和类黄酮含量进行了研究，体外评估了其对 DPPH 自由基清除活性、还原能力，体内测试了其对大鼠 80%乙醇诱导溃疡的胃保护作用。ORE 的植物化学测试对酚类和类黄酮含量呈阳性。ORE 的 DPPH 自由基清除活性和还原能力的 EC（50）分别为 118.65±2.51μg/ml 和 300μg/ml。体内，给予雷尼替丁（50mg/kg）和 ORE 200、400 和 800mg/kg 剂量预处理可显著（p<0.05）降低 80%乙醇诱导的溃疡病变，抑制胃组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、总谷胱甘肽（GSH）的耗竭和髓过氧化物酶（MPO）和丙二醛（MDA）的增加，抑制率分别为 82.68%、49.21%、83.13%和 92.59%，与乙醇组相比。此外，ORE 预处理还可剂量依赖性减轻乙醇引起的组织病理学变化。酚类和类黄酮含量、自由基清除活性和还原能力与 ORE 的抗溃疡特性有关。
