Tembhurne Sachin V, Jagdale Mansi S, Kate Payal, Ar Ziyaurrahman
Department of Pharmacology, AISSMS College of Pharmacy, Kennedy Road, Pune 411001, India.
Pharmacology Department, Allana College of Pharmacy, Pune, India.
Arq Gastroenterol. 2025 Apr 4;62:e24098. doi: 10.1590/S0004-2803.24612024-098. eCollection 2025.
Diarrhea is a gastrointestinal transit disorder and mostly seen in malnourished children's as per WHO report. Malnourished individuals are found to be associated with compromised immunity and lack of nutrients, which makes person susceptible to diarrhoea.
For maintaining the gut health adequate and balance nutrition is essential. In this study, both fermented and non-fermented nutritional supplement was formulated and evaluated against castor-oil induced diarrhoea.
Two groups of rats initially fed a 2% protein-deficient diet for ten weeks. After this period, one group received a diet enriched with nutritional components blended with fermented bovine colostrum, while the other group received a diet with non-fermented nutritional components. At the end of 20th week, castor oil was given to all animals except the control group to induce diarrhoea. Subsequently, these rats were subjected to various assessments including time of onset of the first diarrheal stool (min), faecal weight, faecal score, number of wet stools, intestinal fluid accumulation and Histopathological examination. DPPH radical-scavenging activity of nutritional blend was also determined.
The undernourished rats fed with non-fermented and fermented diet showed delayed the onset of diarrhea and reduction of weight stool, the decrease in the frequency and severity of defecation as well as significantly protected against the intestinal fluid accumulation as compare to negative control groups. The results showed that both the fermented and non-fermented blended composition exhibited antioxidant activity. The intestine of undernourished rats fed with fermented nutritional diet showed the absence of infiltration and improved villi structure.
The study presents promising evidence of the potential benefits of the formulated nutritional compositions in alleviating the castor oil-induced diarrhea in undernourished wistar rats. The antioxidant activity, anti-diarrheal effects and improvements in gut histology suggest that, the nutritional compositions could be explored further as natural interventions for gastrointestinal health.
• Diarrhea is common in malnourished children due to compromised immunity and lack of nutrients.
• Rats were fed a protein-deficient diet (2%) for 10 weeks. One group received fermented bovine colostrum, while the other received non-fermented nutritional components.
• Diarrhea was induced in the animals, and they were evaluated for the onset of diarrhea, fecal weight, frequency, intestinal fluid accumulation, and histology.
• Both supplements delayed the onset of diarrhea, reduced its severity, and exhibited antioxidant activity.
• The nutritional compositions have potential benefits as natural interventions for intestinal health.
腹泻是一种胃肠道转运紊乱疾病，根据世界卫生组织的报告，多见于营养不良的儿童。据发现，营养不良的个体免疫力受损且缺乏营养，这使得他们易患腹泻。
为维持肠道健康，充足且均衡的营养至关重要。在本研究中，制备了发酵型和非发酵型营养补充剂，并针对蓖麻油诱导的腹泻进行评估。
两组大鼠最初喂食2%蛋白质缺乏饮食，持续10周。在此期间后，一组接受富含与发酵牛初乳混合的营养成分的饮食，而另一组接受含有非发酵营养成分的饮食。在第20周结束时，除对照组外，给所有动物喂食蓖麻油以诱导腹泻。随后，对这些大鼠进行各种评估，包括首次腹泻粪便的发作时间（分钟）、粪便重量、粪便评分、稀便次数、肠液积聚和组织病理学检查。还测定了营养混合物的DPPH自由基清除活性。
与阴性对照组相比，喂食非发酵和发酵饮食的营养不良大鼠腹泻发作延迟，粪便重量减轻，排便频率和严重程度降低，并显著防止了肠液积聚。结果表明，发酵型和非发酵型混合组合物均表现出抗氧化活性。喂食发酵营养饮食的营养不良大鼠的肠道显示无浸润且绒毛结构改善。
该研究提供了有前景的证据，表明所制备的营养组合物在减轻营养不良的Wistar大鼠蓖麻油诱导的腹泻方面具有潜在益处。抗氧化活性、抗腹泻作用以及肠道组织学的改善表明，这些营养组合物可作为胃肠道健康的天然干预措施进一步探索。
• 由于免疫力受损和缺乏营养，腹泻在营养不良的儿童中很常见。
• 大鼠喂食2%蛋白质缺乏饮食10周。一组接受发酵牛初乳，而另一组接受非发酵营养成分。
• 诱导动物腹泻，并对它们进行腹泻发作、粪便重量、频率、肠液积聚和组织学评估。
• 两种补充剂均延迟了腹泻发作，降低了其严重程度，并表现出抗氧化活性。
• 这些营养组合物作为肠道健康的天然干预措施具有潜在益处。
