School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Brain Behav Immun. 2010 Nov;24(8):1362-70. doi: 10.1016/j.bbi.2010.07.239. Epub 2010 Jul 16.
Exercise induces mobilisation of CD8(+) T lymphocytes (CD8TL) into the peripheral blood. This response is largely confined to effector-memory CD8TLs: antigen experienced cells which have a strong tissue-homing and effector potential. This study investigated whether effector-memory cells also account for the CD8TL egress from peripheral blood following exercise. As latent Cytomegalovirus (CMV) infection is associated with a robust expansion in the number and proportion of effector-memory CD8TLs, we also investigated if CMV serostatus was a determinant of the CD8TL responses to exercise. Fourteen males (Mean age 35, SD ± 14 yrs), half of whom were CMV seropositive (CMV(+)), ran on a treadmill for 60 min at 80% VO(2) max. Blood was collected at baseline, during the final minute of exercise, and 15 min and 60 min thereafter. CD8TL memory subsets were characterised by flow cytometry, using the cell-surface markers CD45RA, CD27, and CD28. The results confirmed that CD8TLs with an effector-memory phenotype (CD27(-)CD28(-)CD45RA(+/-)) exhibited the largest increase during exercise (+200% to +250%), and also showed the largest egress from blood 60 min post-exercise (down to 40% of baseline values). Strikingly, the mobilisation and subsequent egress of total CD8TLs was nearly twice as large in CMV(+) individuals. This effect appeared specific to CD8TLs, and was not seen for CD4(+) T lymphocytes or total lymphocytes. This effect of CMV serostatus was largely driven by the higher numbers of exercise-responsive effector-memory CD8TLs in the CMV(+) participants. This is the first study to demonstrate that infection history is a determinant of immune system responses to exercise.
运动可引起 CD8(+)T 淋巴细胞（CD8TL）进入外周血。这种反应主要局限于效应记忆性 CD8TL：具有强烈组织归巢和效应潜力的抗原经验细胞。本研究旨在探讨运动后外周血中 CD8TL 的流出是否也与效应记忆细胞有关。由于潜伏性巨细胞病毒（CMV）感染与效应记忆性 CD8TL 数量和比例的显著增加有关，我们还研究了 CMV 血清状态是否是运动后 CD8TL 反应的决定因素。14 名男性（平均年龄 35 岁，标准差 ± 14 岁），其中一半为 CMV 血清阳性（CMV(+))，在跑步机上以 80%的最大摄氧量跑 60 分钟。在基线、运动最后 1 分钟以及运动后 15 分钟和 60 分钟时采集血液。通过流式细胞术，使用细胞表面标志物 CD45RA、CD27 和 CD28 来鉴定 CD8TL 记忆亚群。结果证实，具有效应记忆表型（CD27(-)CD28(-)CD45RA(+/-))的 CD8TL 在运动中表现出最大的增加（增加 200%至 250%），并且在运动后 60 分钟从血液中最大程度地流出（降至基线值的 40%）。引人注目的是，CMV(+)个体中总 CD8TL 的动员和随后的流出量几乎是 CMV(-)个体的两倍。这种效应似乎是 CMV 血清状态的特异性，而在 CD4(+)T 淋巴细胞或总淋巴细胞中未见。CMV 血清状态的这种影响主要是由 CMV(+)参与者中更高数量的运动反应性效应记忆性 CD8TL 引起的。这是第一项证明感染史是运动后免疫系统反应决定因素的研究。
