Lavoy Emily C, Bigley Austin B, Spielmann Guillaume, Rector Jerrald L, Morrison Mark R, O'Connor Daniel P, Simpson Richard J
Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX.
Med Sci Sports Exerc. 2014 Feb;46(2):257-67. doi: 10.1249/MSS.0b013e3182a5a0fb.
Latent cytomegalovirus (CMV) infection has been shown to alter the lymphocyte response to acute aerobic exercise, likely due to the corresponding increase in exercise-responsive memory CD8(+) T cells. It is unknown if latent infection with another herpesvirus, herpes simplex virus 1 (HSV-1), also plays a role in shaping the lymphocyte response to exercise.
Thirty-two men (ages 39.3 ± 14.7 yr) counterbalanced by CMV and HSV-1 serostatus (positive/negative) cycled for 30 min at ∼80% peak power. Blood sampled before, immediately after, and 1 h after exercise was analyzed by flow cytometry for T-cell subset enumeration.
In resting blood, HSV-1(+) had fewer lymphocytes, CD4(+) T cells, KLRG1(-) CD28(+) CD4(+) T cells, and CD45RA(-)CCR7(+)CD4(+) T cells than HSV-1(-), whereas CMV(+) had increased numbers of lymphocytes, CD8(+) T cells, KLRG1(+)CD28(-)CD4(+) and CD8(+) T cells, and CD45RA(+)CCR7(-)CD8(+) T cells and a lower CD4:CD8 T-cell ratio than CMV(-). After exercise, CMV(+) had a greater mobilization of CD8(+) T cells, KLRG1+CD28(-)CD4+ and CD8(+) T cells, and CD45RA+CCR7(-)CD8+ T cells independently of HSV-1 serostatus, as well as a greater egress of these subsets 1 h after exercise. HSV serostatus did not influence total CD8(+) T-cell response to exercise.
The impact of latent CMV infection on the redeployment of T-cell subsets with exercise is independent of HSV-1 infection. This is most likely due to the unique ability of CMV to alter the composition of the memory T-cell pool in favor of exercise-responsive T-cell subsets.
潜伏性巨细胞病毒（CMV）感染已被证明会改变淋巴细胞对急性有氧运动的反应，这可能是由于运动反应性记忆CD8(+) T细胞相应增加所致。尚不清楚另一种疱疹病毒单纯疱疹病毒1型（HSV-1）的潜伏感染是否也在塑造淋巴细胞对运动的反应中起作用。
32名男性（年龄39.3±14.7岁）根据CMV和HSV-1血清学状态（阳性/阴性）进行平衡，以约80%的峰值功率骑行30分钟。在运动前、运动后即刻和运动后1小时采集血液样本，通过流式细胞术分析T细胞亚群计数。
在静息血液中，HSV-1(+)的淋巴细胞、CD4(+) T细胞、KLRG1(-) CD28(+) CD4(+) T细胞和CD45RA(-)CCR7(+)CD4(+) T细胞比HSV-1(-)少，而CMV(+)的淋巴细胞、CD8(+) T细胞、KLRG1(+)CD28(-)CD4(+)和CD8(+) T细胞以及CD45RA(+)CCR7(-)CD8(+) T细胞数量增加，且CD4:CD8 T细胞比值低于CMV(-)。运动后，CMV(+)的CD8(+) T细胞、KLRG1+CD28(-)CD4+和CD8(+) T细胞以及CD45RA+CCR7(-)CD8+ T细胞的动员量更大，且与HSV-1血清学状态无关，运动后1小时这些亚群的流出量也更大。HSV血清学状态不影响总CD8(+) T细胞对运动的反应。
潜伏性CMV感染对运动时T细胞亚群重新分布的影响独立于HSV-1感染。这很可能是由于CMV具有独特的能力来改变记忆T细胞库的组成，使其有利于运动反应性T细胞亚群。
