Department of Experimental Immunology, Sanquin Research, Landsteiner Laboratory, Department of Medical Microbiology, part of the Center for Infectious Diseases and Immunity Amsterdam (CINIMA) at the Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands.
Virology. 2010 Sep 30;405(2):492-504. doi: 10.1016/j.virol.2010.06.029. Epub 2010 Jul 17.
The HIV-1 quasispecies in peripheral blood mononuclear cells (PBMC) is considered to be a mix of actively replicating, latent, and archived viruses and may be genetically distinct from HIV-1 variants in plasma that are considered to be recently produced. Here we analyzed the genetic relationship between gp160 env sequences from replication competent clonal HIV-1 variants that were isolated from PBMC and from contemporaneous HIV-1 RNA in serum and HIV-1 proviral DNA in PBMC of four longitudinally studied therapy naïve HIV-1 infected individuals. Replication competent clonal HIV-1 variants, HIV-1 RNA from serum, and HIV-1 proviral DNA from PBMC formed a single virus population at most time points analyzed. However, an under-representation in serum of HIV-1 sequences with predicted CXCR4 usage was sometimes observed implying that the analysis of viral sequences from different sources may provide a more complete assessment of the viral quasispecies in peripheral blood in vivo.
外周血单核细胞（PBMC）中的 HIV-1 准种被认为是活跃复制、潜伏和存档病毒的混合物，可能与被认为是最近产生的血浆中的 HIV-1 变体在基因上有所不同。在这里，我们分析了从四个纵向研究的未接受治疗的 HIV-1 感染个体的 PBMC 中分离出的具有复制能力的克隆 HIV-1 变体的 gp160 env 序列与同时期血清中的 HIV-1 RNA 和 PBMC 中的 HIV-1 前病毒 DNA 之间的遗传关系。在分析的大多数时间点，具有复制能力的克隆 HIV-1 变体、血清中的 HIV-1 RNA 和 PBMC 中的 HIV-1 前病毒 DNA 形成了单一的病毒群体。然而，有时在血清中观察到预测使用 CXCR4 的 HIV-1 序列的代表性不足，这意味着分析来自不同来源的病毒序列可能提供对体内外周血病毒准种的更完整评估。
