双折射对偏振分辨非线性显微镜及胶原二次谐波产生结构成像的影响。

Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging.

作者信息

Brasselet Sophie, Aït-Belkacem Dora, Gasecka Alicja, Munhoz Fabiana, Brustlein Sophie, Brasselet S

机构信息

Institut Fresnel,CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Campus de St Jérôme, 13013 Marseille, France MOSAIC.

出版信息

Opt Express. 2010 Jul 5;18(14):14859-70. doi: 10.1364/OE.18.014859.

DOI:10.1364/OE.18.014859

PMID:20639973

Abstract

We analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments.

摘要

我们分析了分子和生物样本的各向异性对偏振分辨非线性显微镜成像的影响。我们特别展示了双折射对二次谐波产生（SHG）和双光子激发荧光（TPEF）偏振分辨显微镜成像的不利影响，如果不考虑这一影响，可能会导致对样本特性的错误判定，进而造成对读出信息的错误解读。我们提出了一种方法来测量这种双折射，并在非线性偏振分辨实验中考虑这一效应。