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用于胶原蛋白多参数定量表征的无标记多模态偏振敏感光学显微镜。

Label-free multimodal polarization-sensitive optical microscope for multiparametric quantitative characterization of collagen.

作者信息

Yang Lingxiao, Iyer Rishyashring R, Sorrells Janet E, Chaney Eric J, Boppart Stephen A

机构信息

Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, USA.

Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, USA.

出版信息

Optica. 2024 Feb 20;11(2):155-165. doi: 10.1364/optica.505377. Epub 2024 Jan 25.

DOI:10.1364/optica.505377
PMID:40894932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12392525/
Abstract

Collagen is an essential component of biological tissues with a variety of subtypes. To be able to capture these subtypes, fully exploit the polarization-sensitive light-collagen interactions, and provide comprehensive information of collagen, we integrated polarization-sensitive second-harmonic generation (PSHG) microscopy, polarization-sensitive optical coherence microscopy (PSOCM), and two-photon fluorescence lifetime imaging microscopy into a single-source multimodal system in a simultaneous and spatially co-registered configuration. PSOCM information is used in the PSHG numerical model to enable accurate PSHG analysis of unsectioned fresh tissue. This polarization-sensitive multimodal system provides quantitative multiparametric characterization of collagen and facilitates the fundamental understanding of collagen in the unperturbed tissue microenvironment, which can enable future studies into the role of collagen in various diseases.

摘要

胶原蛋白是生物组织的重要组成部分，有多种亚型。为了能够捕获这些亚型，充分利用偏振敏感光与胶原蛋白的相互作用，并提供胶原蛋白的全面信息，我们将偏振敏感二次谐波产生（PSHG）显微镜、偏振敏感光学相干显微镜（PSOCM）和双光子荧光寿命成像显微镜集成到一个单光源多模态系统中，采用同时且空间共配准的配置。PSOCM信息用于PSHG数值模型，以实现对未切片新鲜组织的准确PSHG分析。这种偏振敏感多模态系统提供了胶原蛋白的定量多参数表征，并有助于在未受干扰的组织微环境中对胶原蛋白有基本的了解，这可为未来研究胶原蛋白在各种疾病中的作用提供支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/7ec127e9c5a2/nihms-2078257-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/95fee9ec1769/nihms-2078257-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/25a7d1ace0dc/nihms-2078257-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/3458497789e8/nihms-2078257-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/5478a644ad66/nihms-2078257-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/7ec127e9c5a2/nihms-2078257-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/95fee9ec1769/nihms-2078257-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/25a7d1ace0dc/nihms-2078257-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/3458497789e8/nihms-2078257-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/5478a644ad66/nihms-2078257-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/195a/12392525/7ec127e9c5a2/nihms-2078257-f0005.jpg

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