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Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection.

作者信息

Liu J P, Hsieh Y Z, Wiesler D, Novotny M

机构信息

Department of Chemistry, Indiana University, Bloomington 47405.

出版信息

Anal Chem. 1991 Mar 1;63(5):408-12. doi: 10.1021/ac00005a004.

DOI:10.1021/ac00005a004
PMID:2064006
Abstract

Amino acids and peptides, from both standard solutions and biological samples, were successfully reacted with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde, at low concentration, to form highly fluorescent isoindole derivatives. The formed mixtures are effectively separated by high-performance capillary electrophoresis and their constituents detected by their laser-induced fluorescence signals. The minimum detectable quantities in the low attomole (10(-18) mol) range are encountered.

摘要

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