通过毛细管电泳分离混合物中的碳水化合物衍生物进行超灵敏荧光检测。
Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis.
作者信息
Liu J P, Shirota O, Wiesler D, Novotny M
机构信息
Department of Chemistry, Indiana University, Bloomington 47405.
出版信息
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2302-6. doi: 10.1073/pnas.88.6.2302.
Abstract
Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide solutes were assessed at low attomole levels (0.5 amol for the CBQCA derivative of galactose). The system has shown considerable promise for high-sensitivity analysis of both neutral and amino sugars in glycoproteins. Complex oligosaccharides, isolated from bovine fetuin by hydrazinolysis, were also successfully "mapped."
摘要
还原胺化后的还原单糖和寡糖，通过毛细管电泳/激光诱导荧光分离并检测为它们的3-(4-羧基苯甲酰基)-2-喹啉甲醛（CBQCA）衍生物。在优化条件下，单糖溶质的最低检测量在低阿托摩尔水平（半乳糖的CBQCA衍生物为0.5 amol）进行评估。该系统在糖蛋白中中性糖和氨基糖的高灵敏度分析方面显示出巨大潜力。通过肼解从牛胎球蛋白中分离出的复杂寡糖也成功地进行了“图谱分析”。
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