Liu J P, Shirota O, Novotny M
Department of Chemistry, Indiana University, Bloomington 47405.
Anal Chem. 1991 Mar 1;63(5):413-7. doi: 10.1021/ac00005a005.
3-(4-Carboxybenzoyl)-2-quinolinecarboxaldehyde has been utilized as a precolumn derivatization agent for various amino sugars. Constituents of various biological mixtures can be converted to highly fluorescent isoindole derivatives, separated by high-performance capillary electrophoresis and determined at attomole (10(-18) mol) levels by a laser-induced fluorescence detector. This method has been applied to the analysis of monosaccharides and acid-hydrolyzed polysaccharides. Carbohydrate moieties derived from a glycoprotein were also tagged and determined.
