Zhao J Y, Diedrich P, Zhang Y, Hindsgaul O, Dovichi N J
University of Alberta, Department of Chemistry, Edmonton, Canada.
J Chromatogr B Biomed Appl. 1994 Jul 15;657(2):307-13. doi: 10.1016/0378-4347(94)00015-8.
The six most abundant hexoses found in mammalian carbohydrates were derivatized to serve as standards for an oligosaccharide sequencing project. Glucose, galactose, mannose, and fucose were reductively aminated to produce 1-amino-1-deoxy alditols. Standards for N-acetylglucosamine and N-acetylgalactosamine were synthesized by reducing glucosamine and galactosamine to 2-amino-2-deoxy alditols. The monosaccharides were labeled with 5-carboxytetramethylrhodamine succinimidyl ester. By carefully adjusting the separation buffer, we have separated the sugar derivatives completely. The detection limit is 100 molecules of labeled sugar with a post-column laser-induced fluorescence detection in a sheath flow cuvette.
哺乳动物碳水化合物中发现的六种最丰富的己糖被衍生化，用作寡糖测序项目的标准品。葡萄糖、半乳糖、甘露糖和岩藻糖经还原胺化反应生成1-氨基-1-脱氧糖醇。N-乙酰葡糖胺和N-乙酰半乳糖胺的标准品通过将葡糖胺和半乳糖胺还原为2-氨基-2-脱氧糖醇来合成。单糖用5-羧基四甲基罗丹明琥珀酰亚胺酯进行标记。通过仔细调整分离缓冲液，我们已将糖衍生物完全分离。在鞘流比色皿中进行柱后激光诱导荧光检测时，检测限为100个标记糖分子。
