Mota Maria Paula, Peixoto Francisco M, Soares Jorge F, Figueiredo Pedro A, Leitão José C, Gaivão Isabel, Duarte José A
University of Trás-os-Montes and Alto Douro, Centro de Investigação em Desporto, Saúde e Desenvolvimento Humano, Vila Real, Portugal.
Age (Dordr). 2010 Sep;32(3):337-46. doi: 10.1007/s11357-010-9138-8. Epub 2010 Mar 20.
The aim of this study was to analyze the influence of aerobic fitness (AF) on age-related lymphocyte DNA damage in humans, giving special attention to the role of the mitochondrial respiratory chain and hydrogen peroxide production. Considering age and AF (as assessed by VO(2)max), 66 males (19-59 years old) were classified as high fitness (HF) or low fitness (LF) and distributed into one of the following groups: young adults (19-29 years old), adults (30-39 years old), and middle-aged adults (over 40 years old). Peripheral lymphocytes obtained at rest were used to assess DNA damage (strand breaks and formamidopyrimidine DNA glycosylase (FPG) sites through the comet assay), activity of mitochondrial complexes I and II (polarographically measured), and the hydrogen peroxide production rate (assayed by fluorescence). Results revealed a significant interaction between age groups and AF for DNA strand breaks (F = 8.415, p = .000), FPG sites (F = 11.766, p = .000), mitochondrial complex I activity (F = 7.555, p = .000), and H(2)O(2) production (F = 7.500, p = .000). Except for mitochondrial complex II activity, the age variation of the remaining parameters was significantly attenuated by HF. Considering each AF level, an increase in DNA strand breaks and FPG sites with age (r = 0.655, p = 0.000, and r = 0.738, p = 0.000, respectively) was only observed in LF. Moreover, decreased mitochondrial complex I activity with age (r = -.470, p = .009) was reported in LF. These results allow the conclusion that high AF seems to play a key role in attenuating the biological aging process.
本研究的目的是分析有氧适能（AF）对人类与年龄相关的淋巴细胞DNA损伤的影响，特别关注线粒体呼吸链和过氧化氢产生的作用。根据年龄和AF（通过最大摄氧量（VO₂max）评估），将66名男性（19 - 59岁）分为高适能（HF）或低适能（LF）组，并分配到以下组之一：青年（19 - 29岁）、成年人（30 - 39岁）和中年成年人（40岁以上）。静息状态下获取的外周淋巴细胞用于评估DNA损伤（通过彗星试验检测链断裂和甲酰胺嘧啶DNA糖基化酶（FPG）位点）、线粒体复合物I和II的活性（极谱法测量）以及过氧化氢产生率（荧光法检测）。结果显示，年龄组和AF之间在DNA链断裂（F = 8.415，p = 0.000）、FPG位点（F = （此处原文有误，推测为11.766），p = 0.000）、线粒体复合物I活性（F = 7.555，p = 0.000）和H₂O₂产生（F = 7.500，p = 0.000）方面存在显著交互作用。除线粒体复合物II活性外，HF显著减弱了其余参数随年龄的变化。考虑每个AF水平，仅在LF中观察到DNA链断裂和FPG位点随年龄增加（r = 0.655，p = 0.000和r = 0.738，p = 0.000）。此外，LF中报告了线粒体复合物I活性随年龄降低（r = -0.470，p = 0.009）。这些结果表明，高AF似乎在减弱生物衰老过程中起关键作用。
