Department of Immunology, Basic Medical College, Tianjin Medical University, PR China.
FEBS Lett. 2010 Aug 20;584(16):3525-32. doi: 10.1016/j.febslet.2010.07.022. Epub 2010 Jul 17.
SGs are mRNA containing cytoplasmic structures that are assembled in response to stress. Tudor-SN protein is a ubiquitously expressed protein. Here, Tudor-SN protein was found to physiologically interact with G3BP, which is the marker and effector of SG. The kinetics of the assembly of SGs in the living cells demonstrated that Tudor-SN co-localizes with G3BP and is recruited to the same SGs in response to different stress stimuli. Knockdown of endogenous Tudor-SN did not inhibit the formation of SGs, but retarded the aggregation of small SGs into large SGs. Thus Tudor-SN may not be an initiator as essential as G3BP for the formation of SGs, but affects the aggregation of SGs. These findings identify Tudor-SN as a novel component of SGs.
SGs 是含有细胞质结构的 mRNA，这些结构是在应激反应中组装的。Tudor-SN 蛋白是一种广泛表达的蛋白质。在这里，Tudor-SN 蛋白被发现与 SG 的标志物和效应物 G3BP 生理性相互作用。活细胞中 SG 组装的动力学表明，Tudor-SN 与 G3BP 共定位，并响应不同的应激刺激被招募到相同的 SG 中。内源性 Tudor-SN 的敲低不会抑制 SG 的形成，但会延迟小 SG 聚集成大 SG。因此，Tudor-SN 可能不是 SG 形成所必需的起始因子，如 G3BP 那样重要，但会影响 SG 的聚集。这些发现将 Tudor-SN 确定为 SG 的一个新组成部分。
