Manjarrez-Gutierrez Gabriel, Gonzalez-Ramirez Misael, Boyzo-Montes de Oca Alfonso, Hernandez-Rodriguez Jorge
Laboratory of Molecular Pathology, Cardiology Hospital, National Medical Center (CMN-SXXI), Mexican Institute of Social Security, Mexico City 06725, Mexico.
Int J Dev Neurosci. 2010 Nov;28(7):621-4. doi: 10.1016/j.ijdevneu.2010.07.227. Epub 2010 Jul 17.
The aim of this study was to determine whether intrauterine growth restriction produces an increase of dihydropteridine reductase activity as a compensatory mechanism that maintains the necessary concentration of cofactor, tetrahydrobiopterin, during accelerated brain serotonin biosynthesis. Intrauterine growth-restricted offspring and controls were used. On days 1, 10, 15 and 21 of life, the brainstem was dissected and l-tryptophan, serotonin, tryptophan-5-hydroxylase and dihydropteridine reductase activities were determined. Intrauterine growth-restricted pups showed a significant increase of l-tryptophan, 5-hydroxytryptamine, tryptophan-5-hydroxylase and also dihydropteridine activity in the brainstem in comparison to normal pups. These results confirm that intrauterine growth restriction produces an increase of serotonin biosynthesis in the brainstem. This is accompanied by an increase in dihydropteridine activity that appears to be a compensatory mechanism to maintain sufficient tetrahydrobiopterin for the donation of electrons during the accelerated synthesis of brain serotonin in intrauterine growth-restricted rats.
本研究的目的是确定宫内生长受限是否会导致二氢蝶啶还原酶活性增加，作为一种补偿机制，在脑5-羟色胺生物合成加速过程中维持辅因子四氢生物蝶呤的必要浓度。使用了宫内生长受限的后代和对照组。在出生后第1、10、15和21天，解剖脑干并测定l-色氨酸、5-羟色胺、色氨酸-5-羟化酶和二氢蝶啶还原酶的活性。与正常幼崽相比，宫内生长受限的幼崽脑干中的l-色氨酸、5-羟色胺、色氨酸-5-羟化酶以及二氢蝶啶活性显著增加。这些结果证实，宫内生长受限会导致脑干中5-羟色胺生物合成增加。这伴随着二氢蝶啶活性的增加，这似乎是一种补偿机制，以便在宫内生长受限大鼠脑5-羟色胺加速合成过程中维持足够的四氢生物蝶呤用于电子传递。
