School of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan Province, 450002, China.
N Biotechnol. 2011 Jan 31;28(1):14-8. doi: 10.1016/j.nbt.2010.07.007. Epub 2010 Jul 17.
The study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. The synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (HPLC). According to the reaction of s-6-a catalyzed by fructosyltransferase from Aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, pH, substrate and enzyme concentration in the reaction, were investigated. All results indicated that the fructosyltransferase could catalyze the s-6-a synthesis, and the optimal conditions of fructosyltransferase in reaction were 50°C, pH 6.2, 48h reaction time, 60% sucrose, 1:3 ratio of g-6-a to sucrose and 4.0mg/L concentration of enzyme. This study plays the important role in sucralose synthesis, because it is very cumbersome in the reported methods.
该研究主要调查了果糖基转移酶作用下蔗糖-6-乙酸酯（s-6-a）的合成。s-6-a 的合成反应在蔗糖和葡萄糖-6-乙酸酯（g-6-a）之间进行，并通过高效液相色谱（HPLC）进行鉴定。根据米曲霉果糖基转移酶催化的 s-6-a 反应，研究了反应的影响因素，如反应中 g-6-a 与蔗糖的比例、温度、时间、pH 值、底物和酶浓度。所有结果表明，果糖基转移酶可以催化 s-6-a 的合成，反应中果糖基转移酶的最佳条件为 50°C、pH6.2、48h 反应时间、60%蔗糖、g-6-a 与蔗糖的比例为 1:3 和 4.0mg/L 的酶浓度。由于在已报道的方法中非常繁琐，因此该研究在三氯蔗糖合成中具有重要意义。
