Wei Tao, Yu Xuan, Wang Yingying, Zhu Yonghua, Du Congcong, Jia Chunxiao, Mao Duobin
School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, Zhengzhou, 450002, People's Republic of China.
Biotechnol Lett. 2014 May;36(5):1015-20. doi: 10.1007/s10529-014-1457-x. Epub 2014 Feb 22.
A novel fructosyltransferase (AoFT) capable of synthesizing sucrose 6-acetate (S6A) from sucrose and glucose 6-acetate has been purified to homogeneity from Aspergillus oryzae ZZ-01. Its molecular mass was ~50 kDa by SDS-PAGE; optimal activity was at 45 °C and it was stable from pH 4.5 to 7.5 with an optimum pH of 6. Mg(2+), K(+) (5 mM), propanol, toluene (50%, v/v), Tween 20 or Triton X-100 (1%, w/v) increased the transfructosylation activity by 20, 17, 17, 10, 25 and 20%, respectively. An overall conversion of 32% was achieved under optimal conditions over 24 h. This is the first report that the purified and characterized the fructosyltransferase from Aspergillus capable of synthesis of S6A from sucrose and glucose 6-acetate.
一种能够利用蔗糖和6-乙酸葡萄糖合成蔗糖6-乙酸酯（S6A）的新型果糖基转移酶（AoFT）已从米曲霉ZZ-01中纯化至均一。通过SDS-PAGE测定其分子量约为50 kDa；最佳活性温度为45℃，在pH 4.5至7.5范围内稳定，最适pH为6。Mg(2+)、K(+)（5 mM）、丙醇、甲苯（50%，v/v）、吐温20或曲拉通X-100（1%，w/v）分别使转果糖基化活性提高20%、17%、17%、10%、25%和20%。在最佳条件下24小时内实现了32%的总转化率。这是首次报道从米曲霉中纯化并鉴定出能够利用蔗糖和6-乙酸葡萄糖合成S6A的果糖基转移酶。
