Ahmed Omar Lawin, Nafee Darogha Suhaila
Department of Biology, College of Education, Salahaddin University-Erbil, Erbil City, Kurdistan Region, Iraq.
Rep Biochem Mol Biol. 2024 Jan;12(4):550-565. doi: 10.61186/rbmb.12.4.550.
There is evident inter-individual variability in women's responses to Chlamydial infections and reproductive tract problems. Women's genetic variations within the Interleukin-10 (IL-10) gene have been linked to variances in response to infection. This study was aimed to demonstrate the profound association of IL-10 with infertility and demonstrate the role of IL-10 (-592 C/A rs1800872) and (-1082 A>G rs1800896) single nucleotide polymorphism (SNPs) gene in the susceptibility and severity of a infection.
In this evaluation study, serum IL-10 concentration was measured in 134 women diagnosed with infertility and 50 healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs1800872 and rs1800896 SNPs genes.
Both female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital by PCR was 46.2% in infertile women. The serum concentration of IL10 was lower in infertile women than healthy participants and higher in infertile -positive women compared to infertile -negative in all groups except endometriosis (Endo) infertility. In rs1800872, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs1800896, the AG genotype and G allele show a greater risk for infertility.
Our results confirmed that rs1800872 and rs1800896 gene polymorphisms were associated with an increased risk of infection.
女性对衣原体感染和生殖道问题的反应存在明显的个体差异。白细胞介素-10（IL-10）基因内的女性基因变异与感染反应的差异有关。本研究旨在证明IL-10与不孕症之间的密切关联，并证明IL-10（-592 C/A rs1800872）和（-1082 A>G rs1800896）单核苷酸多态性（SNP）基因在感染易感性和严重程度中的作用。
在这项评估研究中，通过酶联免疫吸附测定（ELISA）测量了134名被诊断为不孕症的女性和50名健康志愿者的血清IL-10浓度。采用四引物扩增阻滞突变系统-PCR（T-ARMS-PCR）分析来检测rs1800872和rs1800896 SNP基因的基因分型。
两组女性抗衣原体IgM抗体均为阳性，但病例之间的反应强度有所不同。同时，通过PCR检测，不孕症女性的生殖道感染发生率为46.2%。除子宫内膜异位症（Endo）不孕症外，所有组中不孕症女性的血清IL10浓度均低于健康参与者，且衣原体阳性不孕症女性高于衣原体阴性不孕症女性。在rs1800872中，CA基因型和C等位基因与不孕症风险增加相关，但多囊卵巢综合征（PCOS）除外，其为A等位基因。在rs1800896中，AG基因型和G等位基因显示出更高的不孕症风险。
我们的结果证实，rs1800872和rs1800896基因多态性与感染风险增加有关。
