Int J Immunopathol Pharmacol. 2010 Apr-Jun;23(2):671-5. doi: 10.1177/039463201002300233.
Recent studies have indicated that Toll-like receptor polymorphisms or their impaired signalling, specifically TLR-2 and TLR-4, were correlated with a higher risk for allergy. The purpose of this study is to evaluate the associations of TRL-2 and TRL-4 single nucleotide polymorphisms (SNP) and atopic traits in a cohort of 159 Italian allergic children (102 affected by eczema and 57 by IgE-mediated food allergy) and 147 healthy controls recruited in Rome, Italy. DNA was isolated from the peripheral blood and TLR-2 R753Q/TLR-4 D299G polymorphisms were determined by TaqMan MGB probes using Real-Time PCR technique. In the control group, the TLR-2 polymorphism R753Q had a prevalence of 2.5% while the frequency of the TLR-4 D299G was 12%. None of the 159 allergic patients showed the R753Q SNP. By contrast, 7/57 patients with food allergy (12%) and 6/102 subjects with eczema (6%) carried the TLR-4 mutation. In our cohort, no evidence of correlation between TLR-2 or TLR-4 polymorphism and eczema and food allergy incidence and/or severity was found. Further studies are needed to clarify the possible role of TLR-2 and TLR-4 polymorphism in allergic disease, in Italian children.
最近的研究表明，Toll 样受体多态性或其信号转导受损，特别是 TLR-2 和 TLR-4，与过敏风险增加有关。本研究旨在评估 TLR-2 和 TLR-4 单核苷酸多态性（SNP）与意大利罗马招募的 159 名过敏性儿童（102 名患有湿疹，57 名患有 IgE 介导的食物过敏）和 147 名健康对照者之间的关联。从外周血中分离 DNA，并使用 TaqMan MGB 探针通过实时 PCR 技术确定 TLR-2 R753Q/TLR-4 D299G 多态性。在对照组中，TLR-2 多态性 R753Q 的患病率为 2.5%，而 TLR-4 D299G 的频率为 12%。在 159 名过敏患者中，没有一人表现出 R753Q SNP。相比之下，7/57 名食物过敏患者（12%）和 6/102 名湿疹患者（6%）携带 TLR-4 突变。在我们的队列中，没有发现 TLR-2 或 TLR-4 多态性与湿疹和食物过敏的发生和/或严重程度之间存在相关性的证据。需要进一步的研究来阐明 TLR-2 和 TLR-4 多态性在意大利儿童过敏性疾病中的可能作用。
