Kawahara Kazuyoshi, Tanaka Atsushi, Yoon Jaewoo, Yokota Akira
Department of Applied Material and Life Science, College of Engineering, Kanto Gakuin University, 1-50-1 Mutsuura-higashi, Yokohama, Kanagawa, Japan.
J Gen Appl Microbiol. 2010 Jun;56(3):249-55. doi: 10.2323/jgam.56.249.
A parathione-degrading bacterium isolated from rice field in the Philippines, Flavobacterium sp. ATCC 27551 (Sethunathan and Yoshida, 1973, Can. J. Microbiol., 19, 873-875), was re-examined chemotaxonomically and phylogenetically. The strain contained 2-hydroxymyristic acid (2-OH 14 : 0), cis-vaccenic acid (18 : 1 omega7c), and palmitic acid (16 : 0) as major cellular fatty acids, two kinds of glycosphingolipids, and ubiquinone-10 as a sole quinone component. The G+C content of genomic DNA of the strain was 65.9 mol%. The phylogenetic analyses of the 16S rRNA gene indicated that the strain was included in the family Sphingomonadaceae, and most closely related to Sphingobium fuliginis (98.0% similarity) and Sphingobium herbicidovorans (97.3%). The strain showed similar physiological characteristics and a moderate value of DNA-DNA relatedness to S. fuliginis. These data suggested it reasonable to conclude that strain ATCC 27551 was identified as S. fuliginis.
从菲律宾稻田分离出的一种降解对硫磷的细菌，即黄杆菌属菌株ATCC 27551（Sethunathan和Yoshida，1973年，《加拿大微生物学杂志》，19卷，873 - 875页），进行了化学分类学和系统发育学的重新研究。该菌株含有2 - 羟基肉豆蔻酸（2 - OH 14 : 0）、顺式vaccenic酸（18 : 1 ω7c）和棕榈酸（16 : 0）作为主要细胞脂肪酸，两种糖鞘脂，以及泛醌 - 10作为唯一的醌成分。该菌株基因组DNA的G + C含量为65.9摩尔%。16S rRNA基因的系统发育分析表明，该菌株属于鞘脂单胞菌科，与暗黑鞘脂菌（相似性98.0%）和除草剂鞘脂菌（相似性97.3%）关系最为密切。该菌株与暗黑鞘脂菌表现出相似的生理特征以及适度的DNA - DNA相关性值。这些数据表明，得出菌株ATCC 27551被鉴定为暗黑鞘脂菌的结论是合理的。
