Xu Jinghong, Liu Youshan, Tan Weiqiang, Hong Xiaoya
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Jun;27(3):600-5.
To explore the effect of PluronicF-127 and Vascular endthelial growth factor(VEGF) delivery system on the survival of the grafted fat, we divided fat harvest under the same condition into 4 groups. One group served as blank control; the other 3 groups served for experiments with respective to DMEM containing 20 ng/ml VEGF; DMEM containing 30% Pluronic F-127; DMEM containing 20 ng/ml VEGF and 30% Pluronic F-127, and then we transplanted the 4 groups of fat, subcutaneously, on the back of 3 groups of BALB/c nude mice (8 mice per group; injecting 4 points per mouse; 0.2 ml per point). At 3 weeks, 6 weeks and 12 weeks, we dissected the fat grafts, measured their weight retention, and put them in histopathologic examination with the use of HE and CD34 staining. And we compared the weight retention and microvessel density (MVD) of each experiment group versus those of control group. The relation between adipose cell and PluronicF-127 was observed through electron microscope. The results reveal that the MVD and weight of pluronicF-127 and VEGF of the experiment groups are significantly greater than those of other groups. The PluronicF-127 and VEGF composite delivery system can significantly improve the blood circulation for fat transplantation, and increase the survival rate of grafted fat.
为探讨普朗尼克F-127与血管内皮生长因子（VEGF）递送系统对移植脂肪存活的影响，我们将在相同条件下采集的脂肪分为4组。一组作为空白对照；其他3组分别用于含20 ng/ml VEGF的DMEM、含30%普朗尼克F-127的DMEM、含20 ng/ml VEGF和30%普朗尼克F-127的DMEM的实验，然后将这4组脂肪皮下移植到3组BALB/c裸鼠背部（每组8只小鼠；每只小鼠注射4个点；每个点0.2 ml）。在3周、6周和12周时，我们解剖脂肪移植物，测量其重量保留情况，并使用HE和CD34染色进行组织病理学检查。我们比较了各实验组与对照组的重量保留情况和微血管密度（MVD）。通过电子显微镜观察脂肪细胞与普朗尼克F-127之间的关系。结果显示，实验组中普朗尼克F-127和VEGF的MVD和重量显著高于其他组。普朗尼克F-127与VEGF复合递送系统可显著改善脂肪移植的血液循环，提高移植脂肪的存活率。
