Craft Randall O, Rophael John, Morrison Wayne A, Vashi Aditya V, Mitchell Geraldine M, Penington Anthony J
Bernard O'Brien Institute of Microsurgery, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
J Plast Reconstr Aesthet Surg. 2009 Feb;62(2):235-43. doi: 10.1016/j.bjps.2007.11.017. Epub 2008 Feb 21.
Autogenous fat injection is widely used for the correction of acquired and congenital soft tissue defects. However, the high absorption rate results in the need for over-correction of the defect and repeat procedures. We hypothesised that platelet-derived growth factor (PDGF), a potent mitogen and known stimulant for murine preadipocytes, would improve fat graft survival when concentrations were sustained with a gelatine microsphere delivery system.
Abdominal fat was harvested from an otherwise healthy 43-year-old woman during a breast reconstruction. Prior to subdermal injection into severe combined immunodeficient (SCID) mice, the fat grafts were divided into 1-ml aliquots, mixed with microspheres bound to PDGF, free PDGF, or nothing depending on its experimental group, and weighed. The following experimental groups were thus created (minimum n=8 per group): (1) fat graft control, (2) fat graft with free PDGF, (3) fat graft with blank microspheres, and (4) fat graft with microspheres bound to PDGF. After 12 weeks, the fat xenografts were harvested for analysis of weight maintenance and histological and morphometric evaluation.
The addition of PDGF bound to gelatine microspheres was effective in improving xenograft weight maintenance (P=0.018) and preservation of adipose tissue architecture (P<0.0005) compared to controls at 3 months. The microspheres were completely absorbed at 12 weeks.
Sustained, local delivery of PDGF via a gelatine microsphere delivery system resulted in improved weight maintenance of the xenografts with greater preservation of adipose tissue architecture at 3 months compared to controls.
自体脂肪注射广泛用于矫正后天性和先天性软组织缺损。然而，高吸收率导致需要对缺损进行过度矫正并重复手术。我们推测，血小板衍生生长因子（PDGF）是一种强效促细胞分裂剂，也是已知的小鼠前脂肪细胞刺激剂，当通过明胶微球递送系统维持其浓度时，可提高脂肪移植的存活率。
在一位43岁健康女性进行乳房重建期间采集腹部脂肪。在将脂肪移植皮下注射到严重联合免疫缺陷（SCID）小鼠体内之前，将脂肪移植物分成1毫升的等分试样，根据其实验组分别与结合有PDGF的微球、游离PDGF或不添加任何物质混合，并称重。由此创建了以下实验组（每组至少n = 8）：（1）脂肪移植对照组，（2）含游离PDGF的脂肪移植组，（3）含空白微球的脂肪移植组，以及（4）含结合有PDGF的微球的脂肪移植组。12周后，采集脂肪异种移植物以分析重量维持情况以及进行组织学和形态学评估。
与对照组相比，在3个月时，添加结合明胶微球的PDGF可有效改善异种移植物的重量维持情况（P = 0.018）并保留脂肪组织结构（P < 0.0005）。微球在12周时完全被吸收。
与对照组相比，通过明胶微球递送系统持续局部递送PDGF可改善异种移植物的重量维持情况，并在3个月时更好地保留脂肪组织结构。
