Lu Zhengjian, Chen Guoping, Wang Jianhua
College of Bioengineering, Chongqing University, Chongqing 400044, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Jun;27(3):692-5.
Atomic force microscopy is a rather new type of nano microscopic technology. It has some advantages, such as high resolution (sub-nano scale); avoidance of special sample preparation; real-time detection of samples under nearly physiological environment; in situ study of samples under water environment; feasibility of investigating physical and chemical properties of samples at molecular level, etc. In recent years, the application of atomic force microscopy in protein study has brought about outstanding achievements. In this paper are introduced the principle and operation modes of atomic force microscopy, also presented are its application in protein imaging, adsorption, folding-and-unfolding, assembly, and single molecular recognition. Additionally, the future application of atomic force microscopy in protein study is prospected.
原子力显微镜是一种相当新型的纳米显微技术。它具有一些优点，如高分辨率（亚纳米尺度）；无需特殊样品制备；能在近乎生理环境下对样品进行实时检测；可在水环境中对样品进行原位研究；能够在分子水平上研究样品的物理和化学性质等。近年来，原子力显微镜在蛋白质研究中的应用取得了显著成就。本文介绍了原子力显微镜的原理和操作模式，还阐述了其在蛋白质成像、吸附、折叠与去折叠、组装及单分子识别方面的应用。此外，对原子力显微镜在蛋白质研究中的未来应用进行了展望。
