原子力显微镜：蛋白质的机械展开

Atomic force microscopy: mechanical unfolding of proteins.

作者信息

Rounsevell Ross, Forman Julia R, Clarke Jane

机构信息

Department of Chemistry, University of Cambridge, MRC Centre for Protein Engineering, Lensfield Road, Cambridge CB2 1EW, UK.

出版信息

Methods. 2004 Sep;34(1):100-11. doi: 10.1016/j.ymeth.2004.03.007.

DOI:10.1016/j.ymeth.2004.03.007

PMID:15283919

Abstract

Mechanical unfolding of proteins using atomic force microscopy is becoming a routine biophysical technique. Mechanistic investigations in this rapidly evolving field are beginning to resolve the factors that contribute to the behaviour of biological macromolecules under force. Here we describe the force-mode apparatus, the experimental set-up, tractable systems of study, and the analysis of the resultant force data. Finally we summarise some of the recent achievements and limitations of this technique.

摘要

使用原子力显微镜对蛋白质进行机械展开正成为一种常规的生物物理技术。在这个快速发展的领域中进行的机理研究开始解析在力作用下促成生物大分子行为的因素。在此，我们描述了力模式仪器、实验装置、易于处理的研究系统以及对所得力数据的分析。最后，我们总结了该技术最近的一些成就和局限性。