Laurberg P
Horm Metab Res. 1978 Mar;10(2):152-5. doi: 10.1055/s-0028-1093464.
In order to try to characterize the sequence of processes leading to hormone secretion from the stimulated thyroid, the effect of cyclic 3'5' adenosine monophosphate (cAMP) and related compounds were examined in 15 two-sided perfusions of canine thyroids isolated in situ. T4 and T3 concentrations in the effluent were measured radioimmunologically. cAMP 5 mM and TSH 100 muU per ml induced the same pattern of hormone release from the thyroid. After a latency period of 15--25 minutes a steep increase occurred in both T4 and T3 release. During the initial part of the stimulation the rise in T4 relase was somewhat slower than that of T3 release. The prolonged latency period before response earlier recorded in the same preparation during infusions of low concentrations of TSH was not observed during infusions of decreasing concentrations of cAMP (1, 0.8, 0.5 and 0.2 mM) or theophylline (5 and 1 mM). Either there was no response or the latency period was of the same length as that observed after a strong stimulus. These findings suggest that the latency period can be divided in two parts: () a variable, dose dependent satency period confined to the early part of the process sequence leading to secretion--i.e. before cAMP exerts its effect, and 2) an obligatory latency period related to the processes taking place after the formation of pseudopods. The duration of these late processes seems to be independent of the degree of stimulation.
为了试图描述刺激甲状腺后导致激素分泌的一系列过程，在15次对原位分离的犬甲状腺进行的双侧灌注实验中，研究了环磷酸腺苷（cAMP）及相关化合物的作用。用放射免疫法测定流出液中T4和T3的浓度。5 mM的cAMP和每毫升100微单位的促甲状腺激素（TSH）诱导甲状腺释放激素的模式相同。在15 - 25分钟的潜伏期后，T4和T3的释放量急剧增加。在刺激初期，T4释放量的增加比T3释放量的增加稍慢。在输注低浓度TSH时，之前在同一实验制剂中记录到的较长反应潜伏期，在输注浓度逐渐降低的cAMP（1、0.8、0.5和0.2 mM）或茶碱（5和1 mM）时未观察到。要么没有反应，要么潜伏期与强刺激后观察到的相同。这些发现表明潜伏期可分为两部分：（1）一个可变的、剂量依赖性潜伏期，局限于导致分泌的过程序列的早期部分——即在cAMP发挥作用之前，以及（2）一个与伪足形成后发生的过程相关的强制性潜伏期。这些后期过程的持续时间似乎与刺激程度无关。
