Medizinische Klinik III, Universität Heidelberg, 69120 Heidelberg, Germany.
Mediators Inflamm. 2010;2010:194896. doi: 10.1155/2010/194896. Epub 2010 Jun 21.
The Chinese extract Rhizoma coptidis is well known for its anti-inflammatory, antioxidative, antiviral, and antimicrobial activity. The exact mechanisms of action are not fully understood.
We examined the effect of the extract and its main compound, berberine, on LPS-induced inflammatory activity in a murine macrophage cell line. RAW 264.7 cells were stimulated with LPS and incubated with either Rhizoma coptidis extract or berberine. Activation of AP-1 and NFkappaB was analyzed in nuclear extracts, secretion of MCP-1/CCL2 was measured in supernatants.
Incubation with Rhizoma coptidis and berberine strongly inhibited LPS-induced monocyte chemoattractant protein (MCP)-1 production in RAW cells. Activation of the transcription factors AP-1 and NFkappaB was inhibited by Rhizoma coptidis in a dose- and time-dependent fashion.
Rhizoma coptidis extract inhibits LPS-induced MCP-1/CCL2 production in vitro via an AP-1 and NFkappaB-dependent pathway. Anti-inflammatory action of the extract is mediated mainly by its alkaloid compound berberine.
黄连提取物以其抗炎、抗氧化、抗病毒和抗菌活性而闻名。其确切的作用机制尚不完全清楚。
我们研究了提取物及其主要成分小檗碱对脂多糖诱导的小鼠巨噬细胞系炎症活性的影响。用 LPS 刺激 RAW 264.7 细胞,并与黄连提取物或小檗碱孵育。在核提取物中分析 AP-1 和 NFkappaB 的激活,在上清液中测量单核细胞趋化蛋白 1/CCL2 的分泌。
黄连和小檗碱孵育可强烈抑制 LPS 诱导的 RAW 细胞单核细胞趋化蛋白 1(MCP)-1 的产生。黄连以剂量和时间依赖的方式抑制转录因子 AP-1 和 NFkappaB 的激活。
黄连提取物通过依赖于 AP-1 和 NFkappaB 的途径抑制 LPS 诱导的 MCP-1/CCL2 在体外的产生。提取物的抗炎作用主要由其生物碱化合物小檗碱介导。