Department of Clinical Neuroscience, Miyazaki University, Kiyotake, Miyazaki, Japan.
Neurochem Res. 2010 Oct;35(10):1510-5. doi: 10.1007/s11064-010-0204-5. Epub 2010 Jul 22.
This study explored the effect of coral calcium hydride (CCH) on rat intrahippocampal antioxidant ability by measuring the PCAM nitroxide radical decay ratio when CCH was (a) co-perfused into the hippocampus and (b) fed orally to the rats for 4 weeks under a freely moving state. Estimation of the in vivo antioxidant effect was obtained by administration of the blood-brain barrier-permeable PCAM nitroxide radical and the measured PCAM radical decay ratio then correlated to the amount of antioxidant in the brain using electron spin resonance (ESR) spectroscopy combined with microdialysis. The half-life periods of PCAM in rats treated with CCH in both the co-perfusion and orally fed groups were significantly shorter compared to the control group. These results clarify the mechanism that CCH may exert antioxidant activity by significantly enhancing the basal endogenous antioxidant ability in the hippocampus through a synergistic effect with α-tocopherol and ascorbic acid.
本研究通过测量当珊瑚钙氢化物（CCH）（a）共灌注到海马体中和（b）在自由活动状态下口服给予大鼠 4 周时，PCAM 氮氧自由基衰减比，来探讨 CCH 对大鼠海马内抗氧化能力的影响。通过给予血脑屏障可渗透的 PCAM 氮氧自由基，并使用电子自旋共振（ESR）光谱结合微透析来测量 PCAM 自由基衰减比，从而对体内抗氧化作用进行评估。与对照组相比，在共灌注和口服给予 CCH 的大鼠中，PCAM 的半衰期明显缩短。这些结果阐明了 CCH 可能通过与α-生育酚和抗坏血酸协同作用，显著增强海马内基础内源性抗氧化能力，从而发挥抗氧化活性的机制。
