MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
School of Medicine, Hangzhou Normal University, Hangzhou 311121, China.
Biosensors (Basel). 2021 Jun 30;11(7):216. doi: 10.3390/bios11070216.
The excess of low-density lipoprotein (LDL) strongly promotes the accumulation of cholesterol on the arterial wall, which can easily lead to the atherosclerotic cardiovascular diseases (ACDs). It is a challenge on how to recognize and quantify the LDL with a simple and sensitive analytical technology. Herein, β-cyclodextrins (β-CDs), acting as molecular receptors, can bind with LDL to form stable inclusion complexes via the multiple interactions, including electrostatic, van der Waals forces, hydrogen bonding and hydrophobic interactions. With the combination of gold nanoparticles (Au NPs) and β-CDs, we developed an electrochemical sensor providing an excellent molecular recognition and sensing performance towards LDL detection. The LDL dynamic adsorption behavior on the surface of the β-CD-Au electrode was explored by electrochemical impedance spectroscopy (EIS), displaying that the electron-transfer resistance (Ret) values were proportional to the LDL (positively charged apolipoprotein B-100) concentrations. The β-CD-Au modified sensor exhibited a high selectivity and sensitivity (978 kΩ·µM) toward LDL, especially in ultra-low concentrations compared with the common interferers HDL and HSA. Due to its excellent molecular recognition performance, β-CD-Au can be used as a sensing material to monitor LDL in human blood for preventing ACDs in the future.
过量的低密度脂蛋白 (LDL) 强烈促进胆固醇在动脉壁上的积累，这很容易导致动脉粥样硬化性心血管疾病 (ACDs)。如何用简单而敏感的分析技术识别和定量 LDL 是一个挑战。在这里，β-环糊精 (β-CDs) 作为分子受体，可以通过多种相互作用（包括静电、范德华力、氢键和疏水相互作用）与 LDL 形成稳定的包合复合物。我们结合金纳米粒子 (Au NPs) 和 β-CDs，开发了一种电化学传感器，该传感器对 LDL 检测具有出色的分子识别和传感性能。通过电化学阻抗谱 (EIS) 研究了 LDL 在β-CD-Au 电极表面的动态吸附行为，结果表明电子转移电阻 (Ret) 值与 LDL（带正电荷的载脂蛋白 B-100）浓度成正比。与常见的干扰物 HDL 和 HSA 相比，β-CD-Au 修饰的传感器对 LDL 具有高选择性和灵敏度（978 kΩ·µM），尤其是在超低浓度下。由于其出色的分子识别性能，β-CD-Au 可用作传感材料来监测人体血液中的 LDL，以预防未来的 ACDs。
