Li Lian, Zheng Jin-Wei, Hang Bao-Jian, Doronina Nina V, Trotsenko Yuri A, He Jian, Li Shun-Peng
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1561-1566. doi: 10.1099/ijs.0.020925-0. Epub 2010 Jul 23.
The taxonomic status was determined of an aerobic, facultatively methylotrophic strain, JZL-4(T), isolated from activated sludge. The cells were gram-negative, asporogenous, colourless, motile, short rods. The strain utilized methanol, methylamine, formate and a variety of polycarbon compounds, but not methane, dichloromethane or CO(2)/H(2), as carbon and energy sources. C(1) compounds were assimilated via the isocitrate lyase-negative serine pathway. Optimal growth occurred at 30 °C, pH 6.5-7.5 and 0.5 % (w/v) NaCl. The major cellular fatty acids were C(18 : 1)ω7c and C(18 : 0). The major phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine (PME); PME, the main phospholipid of strain JZL-4(T), was absent or present in only minor amounts in Methylopila capsulata IM1(T), Methylopila helvetica DM9(T) and Albibacter methylovorans DM10(T). The major ubiquinone was Q-10. The DNA G+C content of strain JZL-4(T) was 70.4 mol% (T(m)). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain showed high sequence similarities to M. capsulata IM1(T) (97.2 %), A. methylovorans DM10(T) (94.9 %) and M. helvetica DM9(T) (94.1 %), and showed less than 94 % similarity to strains of other species with validly published names. Strain JZL-4(T) had a low level of DNA-DNA relatedness (34 %) with M. capsulata IM1(T). On the basis of phenotypic, genetic and phylogenetic data, strain JZL-4(T) is proposed to represent a novel species of the genus Methylopila, with the name Methylopila jiangsuensis sp. nov. The type strain is strain JZL-4(T) ( = ACCC 05406(T) = DSM 22718(T) = VKM B-2555(T)).
确定了从活性污泥中分离出的一株好氧兼性甲基营养型菌株JZL-4(T)的分类地位。该菌细胞革兰氏阴性，无芽孢，无色，具运动性，为短杆状。该菌株利用甲醇、甲胺、甲酸和多种聚碳酸化合物作为碳源和能源，但不利用甲烷、二氯甲烷或CO₂/H₂。C₁化合物通过异柠檬酸裂合酶阴性的丝氨酸途径进行同化。最适生长温度为30℃，pH值为6.5 - 7.5，NaCl浓度为0.5%（w/v）。主要细胞脂肪酸为C₁₈:₁ω7c和C₁₈:₀。主要磷脂为磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰甘油和磷脂酰单甲基乙醇胺（PME）；PME是菌株JZL-4(T)的主要磷脂，在荚膜甲基螺菌IM1(T)、瑞士甲基螺菌DM9(T)和食甲基苍白杆菌DM10(T)中不存在或仅少量存在。主要泛醌为Q-10。菌株JZL-4(T)的DNA G+C含量为70.4 mol%（Tm）。基于16S rRNA基因序列的系统发育分析表明，该菌株与荚膜甲基螺菌IM1(T)（97.2%）、食甲基苍白杆菌DM10(T)（94.9%）和瑞士甲基螺菌DM9(T)（94.1%）具有较高的序列相似性，与其他有效发表名称的物种的菌株相似性低于94%。菌株JZL-4(T)与荚膜甲基螺菌IM1(T)的DNA-DNA相关性较低（34%）。基于表型、遗传和系统发育数据，菌株JZL-4(T)被提议代表甲基螺菌属的一个新物种，命名为江苏甲基螺菌（Methylopila jiangsuensis sp. nov.）。模式菌株为菌株JZL-4(T)（=ACCC 05406(T)=DSM 22718(T)=VKM B-2555(T)）。
