IBPM RAS, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences, Federal Research Center Pushchino Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki, 5, Pushchino, Moscow Region, Russia, 142290.
Institute of Cell Biophysics of the Russian Academy of Sciences, Federal Research Center Pushchino Center for Biological Research, Russian Academy of Sciences, Institutskaya, 3, Pushchino, Moscow Region, Russia, 142290.
Antonie Van Leeuwenhoek. 2019 Sep;112(9):1307-1316. doi: 10.1007/s10482-019-01263-x. Epub 2019 Apr 10.
An aerobic facultatively methylotrophic bacterium, designated strain Das4.1, was isolated from a root of Daucus carota L. The cells of this strain were observed to be Gram-stain negative, asporogenous, non-motile short rods multiplying by binary fission. Strain Das4.1 can utilise methanol, methylamine and a variety of polycarbon compounds as carbon and energy sources. C-compounds were found to be assimilated via the isocitrate lyase-negative variant of the serine pathway. On medium with 0.5% methanol, growth of strain Das4.1 was observed at pH 5.5-9.0 (optimum, pH 6.0-7.0) and 18-37 °C (optimum, 24-29 °C) and in the presence of 0-2% (w/v) NaCl (optimum, 0.05%). Cells are catalase and oxidase positive and synthesise indole from L-tryptophan. The major fatty acids of methanol-grown cells were identified as C, C and 11-methyl-C. The predominant phospholipids were found to be phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The major respiratory quinone was identified as Q-10. The DNA G + C content of strain Das4.1 was determined to be 67.3 mol% (T). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Das4.1 belongs to the genus Methylopila and shows high sequence similarity to Methylopila oligotropha 2395A (98.4%) and Methylopila capsulata IM1 (98.0%). However, the DNA-DNA relatedness of strain Das4.1 with M. oligotropha 2395A was only 22 ± 3%. Based on genotypic, chemotaxonomic and physiological characterisation, the isolate can be classified as a novel species of the genus Methylopila, for which the name Methylopila carotae sp. nov. is proposed. The type strain is Das4.1 (= VKM B-3244 = CCUG 72399).
一株好氧兼性甲基营养型细菌，被命名为 Das4.1 菌株，从胡萝卜（Daucus carota L.）的根部分离得到。该菌株的细胞经革兰氏染色呈阴性，无芽孢，不运动，短杆状，通过二分分裂繁殖。Das4.1 菌株可以利用甲醇、甲胺和多种聚碳酸化合物作为碳源和能源。发现 C 化合物通过丝氨酸途径的异柠檬酸裂解酶阴性变体被同化。在含有 0.5%甲醇的培养基中，Das4.1 菌株在 pH5.5-9.0（最佳，pH6.0-7.0）和 18-37°C（最佳，24-29°C）下生长，并在 0-2%（w/v）NaCl（最佳，0.05%）的存在下生长。细胞呈过氧化氢酶和氧化酶阳性，从 L-色氨酸合成吲哚。甲醇培养细胞的主要脂肪酸为 C、C 和 11-甲基-C。主要的磷脂被鉴定为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和磷脂单甲乙醇胺。主要的呼吸醌为 Q-10。Das4.1 菌株的 DNA G+C 含量为 67.3mol%（T）。基于 16S rRNA 基因序列比较的系统发育分析表明，Das4.1 菌株属于甲基营养菌属，与甲基营养菌寡养单胞菌 2395A（98.4%）和甲基营养菌荚膜菌 IM1（98.0%）具有高序列相似性。然而，Das4.1 菌株与 M. oligotropha 2395A 的 DNA-DNA 相关性仅为 22±3%。基于基因型、化学分类学和生理学特征，该分离株可归类为甲基营养菌属的一个新种，命名为甲基营养菌胡萝卜亚种（Methylopila carotae sp. nov.）。模式菌株为 Das4.1（=VKM B-3244=CCUG 72399）。
