G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State Institute of Natural Sciences, Pushchino, Moscow region 142290, Russia.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1847-1852. doi: 10.1099/ijs.0.042028-0. Epub 2012 Sep 14.
A newly isolated, facultatively methylotrophic bacterium (strain MUSA(T)) was investigated. The isolate was strictly aerobic, Gram-stain-negative, asporogenous, motile, rod-shaped and multiplied by binary fission. The strain utilized methanol, methylamine and an apparently narrow range of multi-carbon compounds, but not methane, dichloromethane or CO2/H2, as the carbon and energy sources. Growth occurred at pH 5.5-9.5 (optimum, pH 7.0) and 16-40 °C (optimum, 28-30 °C). The major fatty acids of methanol-grown cells were C18 : 1ω7c, C18 : 0 and 11-methyl-C18 : 1ω7c . The predominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. The major ubiquinone was Q-10. The strain had methanol and methylamine dehydrogenases as well as the enzymes of the N-methylglutamate pathway (lyases of γ-glutamylmethylamide and N-methylglutamate). C1 assimilation occurs via the isocitrate lyase-negative serine pathway. Ammonium was assimilated by glutamate dehydrogenase and the glutamate cycle (glutamate synthase/glutamine synthetase). The DNA G+C content of the strain was 64.5 mol% (determined from the melting temperature). Based on 16S rRNA gene sequence similarity (97.0-98.9 %) and DNA-DNA relatedness (36-38 %) with representatives of the genus Methylopila (Methylopila capsulata IM1(T) and Methylopila jiangsuensis JZL-4(T)) the isolate was classified as a novel species of the genus Methylopila, for which the name Methylopila musalis sp. nov. is proposed. The type strain is MUSA(T) ( = VKM B-2646(T) = DSM 24986(T) = CCUG 61696(T)).
一株新分离的兼性甲基营养细菌（菌株 MUSA(T)）被研究。该分离株为严格需氧、革兰氏阴性、无孢子、运动、杆状，通过二分分裂繁殖。该菌株利用甲醇、甲胺和显然范围较窄的多碳化合物，但不是甲烷、二氯甲烷或 CO2/H2 作为碳源和能源。生长发生在 pH5.5-9.5（最佳，pH7.0）和 16-40°C（最佳，28-30°C）。甲醇生长细胞的主要脂肪酸为 C18 : 1ω7c、C18 : 0 和 11-甲基-C18 : 1ω7c。主要磷脂为磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰甘油和磷脂酰单甲基乙醇胺。主要的泛醌为 Q-10。该菌株具有甲醇和甲胺脱氢酶以及 N-甲基谷氨酸途径的酶（γ-谷氨酰甲基酰胺和 N-甲基谷氨酸的裂解酶）。C1 的同化通过异柠檬酸裂解酶阴性丝氨酸途径发生。铵通过谷氨酸脱氢酶和谷氨酸循环（谷氨酸合酶/谷氨酰胺合成酶）同化。该菌株的 DNA G+C 含量为 64.5 mol%（根据融解温度确定）。基于 16S rRNA 基因序列相似性（97.0-98.9%）和 DNA-DNA 相关性（36-38%）与甲基营养菌属（Methylopila capsulata IM1(T) 和 Methylopila jiangsuensis JZL-4(T)）的代表，该分离株被分类为甲基营养菌属的一个新种，命名为 Methylopila musalis sp. nov. 。模式菌株为 MUSA(T)（=VKM B-2646(T)=DSM 24986(T)=CCUG 61696(T)）。
