《湖北山楂中的化学成分及其对人癌细胞系的细胞毒活性评价》

Compounds from Viburnum sargentii Koehne and evaluation of their cytotoxic effects on human cancer cell lines.

机构信息

Department of Forest Products and Biotechnology, College of Forest Sciences, Kookmin University, Seoul, 136-702, Korea.

出版信息

Molecules. 2010 Jun 25;15(7):4599-609. doi: 10.3390/molecules15074599.

DOI:10.3390/molecules15074599

PMID:20657380

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6257607/

Abstract

Compounds were isolated from a methanol extract of the dried stem barks of Viburnum sargentii Koehne. The structures of the compounds, namely 9'-O-methylvibsanol (3), furcatoside A (4) and lareciresinol (5) were elucidated by analysis of spectroscopic data and comparison with values for previously known analogues. In addition, (+)-catechin (1), (+)-epicatechin (2) were also isolated. This work also examined the cytotoxic effects of three compounds 3-5 (25-100 microM) in MCF-7 and A549 cells after 24, 48 and 72 h of exposure. Our results showed that 9'-O-methylvibsanol (3) exhibited strong concentration-dependent anticancer effects according to the MTT assay and produced morphological changes consistent with apoptosis, as confirmed by Ho3342 staining analysis revealed that more apoptotic cells were observed after 9'-Omethylvibsanol (3) treatment.

摘要

化合物从 Viburnum sargentii Koehne 的干燥茎皮的甲醇提取物中分离得到。通过分析光谱数据并与以前已知类似物的值进行比较，阐明了化合物的结构，即 9'-O-甲基 Vibsanol（3）、Furcatoside A（4）和Lareciresinol（5）。此外，还分离出了（+）-儿茶素（1）和（+）-表儿茶素（2）。这项工作还研究了三种化合物 3-5（25-100 μM）在 MCF-7 和 A549 细胞中暴露 24、48 和 72 h 后的细胞毒性作用。我们的结果表明，根据 MTT 测定，9'-O-甲基 Vibsanol（3）表现出强烈的浓度依赖性抗癌作用，并产生与凋亡一致的形态变化，这通过 Ho3342 染色分析得到证实，分析显示，在用 9'-O-甲基 Vibsanol（3）处理后，观察到更多的凋亡细胞。