Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Visegradska 33, P.O.Box 224, 18000 Nis, Serbia.
Molecules. 2010 Jun 10;15(6):4213-26. doi: 10.3390/molecules15064213.
The present study is focused on anthocyanin derivatives characterizing the antioxidant activity of Cabernet Sauvignon wines produced from different vineyard regions in the Balkans. These bioactive compounds were quantified with a high performance liquid chromatography (HPLC)-diode array detection (DAD) method. The antiradical activity was estimated by the ability of the wine to scavenge the stable 2,2;-diphenyl-1-picrylhydrazyl free radical (DPPH(*)). The results show that the total anthocyanin content varied from 205.88 to 1940.28 mg/L, depending on agroclimatic factors and the enological practices of the corresponding vineyard region. The most prominent antocyanin in all investigated Cabernet Sauvignon wines was malvidin-3-O-monoglucoside, which accounted for 50.57% of total content, followed by its acetyl derivatives, 15.45%, and p-coumaryl derivatives 5.66%. The relationship between the anthocyanin derivatives and free radical scavenging activity is discussed. A high correlation between total anthocyanin content and DPPH scavenging ability of tested wines was confirmed (r(2) = 0.9619). The significant correlations were obtained between antiradical activity and the sum of 3-monoglucoside (r(2) = 0.95594), the sum of 3-acetyl-3-glucoside (r(2) = 0.9728) and the sum of p-coumaryl-3-glucoside (r(2) = 0.8873) of wine samples. It can be concluded that, the anthocyanin composition can be used as biochemical marker for the authenticity of red grape cultivar and their corresponding single-cultivar wine.
本研究集中于鉴定来自巴尔干半岛不同葡萄园地区酿造的赤霞珠葡萄酒的抗氧化活性的花色苷衍生物。这些生物活性化合物采用高效液相色谱（HPLC）-二极管阵列检测（DAD）方法进行定量。自由基清除能力通过葡萄酒清除稳定的 2,2-二苯基-1-苦基肼自由基（DPPH（*））的能力来估计。结果表明，总花色苷含量根据农业气候因素和相应葡萄园地区的酿造工艺而变化，范围为 205.88 至 1940.28mg/L。在所研究的所有赤霞珠葡萄酒中，最突出的花色苷是矢车菊素-3-O-单葡萄糖苷，占总含量的 50.57%，其次是其乙酰衍生物，占 15.45%，对香豆酰基衍生物占 5.66%。讨论了花色苷衍生物与自由基清除活性之间的关系。证实了测试葡萄酒的总花色苷含量与 DPPH 清除能力之间存在高度相关性（r²=0.9619）。还获得了抗自由基活性与 3-单葡萄糖苷的总和（r²=0.95594）、3-乙酰-3-葡萄糖苷的总和（r²=0.9728）和对香豆酰基-3-葡萄糖苷的总和（r²=0.8873）之间的显著相关性。可以得出结论，花色苷组成可以用作红葡萄品种及其相应单一品种葡萄酒真实性的生化标志物。
