Hong Min Jeong, Kim Dae Yeon, Ahn Joon-Woo, Kang Si-Yong, Seo Yong Weon, Kim Jin-Baek
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
Division of Biotechnology, Korea University, Seoul, Republic of Korea.
Genet Mol Biol. 2018;41(3):611-623. doi: 10.1590/1678-4685-GMB-2017-0189. Epub 2018 Jun 28.
We aimed to investigate the biological responses induced by acute and chronic gamma irradiation in colored wheat seeds rich in natural antioxidants. After acute and chronic irradiation, the phenotypic effects on plant growth, germination rate, seedling height, and root length were examined, and the biochemical changes were investigated by analyzing the expression of antioxidant enzyme-related genes, antioxidant enzyme activities, and total antioxidant capacity. High dosages of chronic radiation reduced plant growth compared with the controls. Electron spin resonance measurement and 2,2-diphenyl-1-picrylhydrazyl activity analysis showed lower amount of free radicals in colored wheat seeds on chronic irradiation with low dosage of gamma rays compared to seeds subjected to acute irradiation. Expression levels of anthocyanin biosynthesis genes, antioxidant-related genes, and antioxidant enzyme activity in seeds and young leaves of seedling showed diverse effects in response to different dosages and types of gamma irradiation. This suggests that phenotype is affected by the dosage and type of gamma radiation, and the phytochemicals in colored wheat seeds involved in antioxidant activity to scavenge free radicals respond differently to irradiation types. This provides evidence that acute and chronic exposure to radiation have different effects on seeds and young leaves after germination.
我们旨在研究富含天然抗氧化剂的有色小麦种子在急性和慢性γ辐射下诱导的生物学反应。在急性和慢性辐射后，检测了对植物生长、发芽率、幼苗高度和根长的表型效应，并通过分析抗氧化酶相关基因的表达、抗氧化酶活性和总抗氧化能力来研究生化变化。与对照相比，高剂量的慢性辐射降低了植物生长。电子自旋共振测量和2,2-二苯基-1-苦基肼活性分析表明，与接受急性辐射的种子相比，低剂量γ射线慢性辐射的有色小麦种子中自由基含量较低。种子和幼苗幼叶中花青素生物合成基因、抗氧化相关基因的表达水平以及抗氧化酶活性在不同剂量和类型的γ辐射下表现出不同的影响。这表明表型受γ辐射剂量和类型的影响，有色小麦种子中参与抗氧化活性以清除自由基的植物化学物质对辐射类型的反应不同。这提供了证据，表明急性和慢性辐射暴露对发芽后的种子和幼叶有不同影响。
