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关于决明子的真实性、含量测定和化学成分分析的标准化。

Standardization of Cassia spectabilis with respect to authenticity, assay and chemical constituent analysis.

机构信息

Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.

出版信息

Molecules. 2010 May 10;15(5):3411-20. doi: 10.3390/molecules15053411.

DOI:10.3390/molecules15053411
PMID:20657490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6257450/
Abstract

Quality control standardizations of the various medicinal plants used in traditional medicine is becoming more important today in view of the commercialization of formulations based on these plants. An attempt at standardization of Cassia spectabilis leaf has been carried out with respect to authenticity, assay and chemical constituent analysis. The authentication involved many parameters, including gross morphology, microscopy of the leaves and functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy. The assay part of standardization involved determination of the minimum inhibitory concentration (MIC) of the extract which could help assess the chemical effects and establish curative values. The MIC of the C. spectabilis leaf extracts was investigated using the Broth Dilution Method. The extracts showed a MIC value of 6.25 mg/mL, independent of the extraction time. The chemical constituent aspect of standardization involves quantification of the main chemical components in C. spectabilis. The GCMS method used for quantification of 2,4-(1H,3H)-pyrimidinedione in the extract was rapid, accurate, precise, linear (R(2) = 0.8685), rugged and robust. Hence this method was suitable for quantification of this component in C. spectabilis. The standardization of C. spectabilis is needed to facilitate marketing of medicinal plants, with a view to promoting the export of valuable Malaysian Traditional Medicinal plants such as C. spectabilis.

摘要

鉴于基于这些植物的制剂的商业化，今天对传统医学中使用的各种药用植物进行质量控制标准化变得越来越重要。我们已经尝试对决明子叶进行标准化，包括真实性、含量测定和化学成分分析。标准化的鉴定涉及许多参数，包括宏观形态、叶片显微镜检查和傅里叶变换红外（FTIR）光谱的官能团分析。标准化的含量测定部分涉及提取物的最小抑菌浓度（MIC）的测定，这有助于评估化学效应并确定治疗值。使用肉汤稀释法研究了决明子叶提取物的 MIC。提取物的 MIC 值为 6.25mg/mL，与提取时间无关。标准化的化学成分方面涉及决明子中主要化学成分的定量。用于提取物中 2,4-(1H,3H)-嘧啶二酮定量的 GCMS 方法快速、准确、精密、线性（R(2) = 0.8685）、坚固耐用。因此，该方法适用于决明子中该成分的定量。需要对决明子进行标准化，以促进药用植物的营销，从而促进有价值的马来西亚传统药用植物（如决明子）的出口。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/ed57f20b0461/molecules-15-03411-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/ed6c3e2ed156/molecules-15-03411-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/6adeae2880b3/molecules-15-03411-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/398c1274ef75/molecules-15-03411-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/3e149476da45/molecules-15-03411-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/ed57f20b0461/molecules-15-03411-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/ed6c3e2ed156/molecules-15-03411-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/6adeae2880b3/molecules-15-03411-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/398c1274ef75/molecules-15-03411-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/3e149476da45/molecules-15-03411-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d16/6257450/ed57f20b0461/molecules-15-03411-g005.jpg

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本文引用的文献

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Further bioactive piperidine alkaloids from the flowers and green fruits of Cassia spectabilis.来自粉叶决明花和绿色果实的更多生物活性哌啶生物碱。
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