Soetardjo Surjani, Jong Poh Chan, Ahmad Mohamad Noor, Lachimanan Yoga Latha, Sreenivasan Sasidharan
School of Distance Education, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia.
Malays J Nutr. 2007 Mar;13(1):81-7. Epub 2007 Mar 15.
The antimicrobial activity of the Centipeda minima L. (Asteraceae) extract was evaluated against seven microorganisms using the disc diffusion method. The extract showed a broad spectrum of antimicrobial activity against all the tested bacterial strains, especially Enterobacter aerogenes, Klebsiella pneumonia, Staphylococcus aureus and Yersinia enterocolitica. The chemical composition of the extract obtained from Centipeda minima was analysed by GC-MS. Twentythree compounds, constituting about 84.75 % of the total extract, were identified. The main constituents were palmitic acid (7.47%), (Z,Z)-9-,12-octadecatrienoic acid (6.52%), (Z,Z,Z)-9-,12-octadecatrienoic acid (7.01%), phytol (7.01%), naptho[2.3-b]furan-2-(3H)-on (6.21%), 1-(1,2,3,4,7,7a-hexahydro-1,4,4,5-tetramethyl-1,3a-ethano-3aH-inden-6-yl)etanon (7.95%), 1,3,5-tri-tertbutyl- benzene (4.52%), (3Z)-2-methyl-3-octen-2-ol (5.95%) and artemisia ketone (4.98%). The extract was also tested against brine shrimp for toxicity. There was no significant toxicity as it only recorded a LC50 value of 4.92 mg/ml. The study shows that the extract is a good antimicrobial agent with potential applications in public health against diseases.
采用纸片扩散法评估了最小叶紫菀（菊科）提取物对七种微生物的抗菌活性。该提取物对所有测试的细菌菌株均表现出广谱抗菌活性，尤其是产气肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌和小肠结肠炎耶尔森菌。采用气相色谱 - 质谱联用仪（GC-MS）分析了从最小叶紫菀中获得的提取物的化学成分。鉴定出23种化合物，约占提取物总量的84.75%。主要成分是棕榈酸（7.47%）、（Z,Z）-9,12-十八碳二烯酸（6.52%）、（Z,Z,Z）-9,12-十八碳三烯酸（7.01%）、叶绿醇（7.01%）、萘并[2.3-b]呋喃-2-(3H)-酮（6.21%）、1-(1,2,3,4,7,7a-六氢-1,4,4,5-四甲基-1,3a-亚乙基-3aH-茚-6-基)乙酮（7.95%）、1,3,5-三叔丁基苯（4.52%）、（3Z）-2-甲基-3-辛烯-2-醇（5.95%）和青蒿酮（4.98%）。还对该提取物进行了卤虫毒性测试。由于其LC50值仅为4.92 mg/ml，因此没有明显毒性。该研究表明，该提取物是一种良好的抗菌剂，在公共卫生领域对抗疾病具有潜在应用价值。
