Scott R Q, Roschger P, Devaraj B, Inaba H
Biophoton Project, Research Development Corporation of Japan (JRDC) Sendai.
FEBS Lett. 1991 Jul 8;285(1):97-8. doi: 10.1016/0014-5793(91)80733-j.
The first thermodynamic measurements of the intensity of light emission associated with native lipid peroxidation in a biological membrane are described. Kinetics of the radical chain reaction are shown to be sensitive to membrane structural phase and lipid dynamics. This is demonstrated by a novel measurement of a phase transition in the membrane of the intact mammalian nucleus. The apparent activation energies of lipid peroxidation in this system are also obtained for the first time. We suggest that this measurement may be more generally applicable as a method for monitoring membrane phase transitions.
本文描述了首次对生物膜中与天然脂质过氧化相关的光发射强度进行的热力学测量。自由基链反应的动力学对膜结构相和脂质动力学敏感。这通过对完整哺乳动物细胞核膜相变的新测量得到了证明。该系统中脂质过氧化的表观活化能也是首次获得。我们认为这种测量作为监测膜相变的方法可能更具普遍适用性。
