Pieri C, Falasca M, Marcheselli F, Recchioni R, Moroni F
Cytology Center, Gerontological Research Department of INRCA, Ancona, Italy.
Cell Mol Biol. 1992 Jul;38(4):437-42.
The effect of peroxidation on 5'-nucleotidase activity as well as on membrane microviscosity has been investigated in liver plasma membranes from Wistar rats. The peroxidation was performed with 100 microM H2O2 and 200 microM FeSO4 and/or with 5 mM t-butylhydroperoxide. Treatment of the membranes with these oxidizing agents resulted in an elevation of the transition temperatures of the polarization of the lipid fluorescent probes 1,6 diphenyl-1,3,5 hexatriene (DPH), 3-p-(6-phenyl) 1,3,5 hexatriene phenylpropionic acid (PA-DPH) as well as of the fluorescent thiol reagent N-(1-pyrene) maleimide (1-PM). The peroxidation resulted in a decrease of the activity of 5'nucleotidase. Our data support that the increase of membrane microviscosity of the lipid domain regulates the activity of 5'-nucleotidase.
研究了过氧化作用对Wistar大鼠肝细胞膜中5'-核苷酸酶活性以及膜微粘度的影响。过氧化作用通过100微摩尔/升过氧化氢和200微摩尔/升硫酸亚铁和/或5毫摩尔叔丁基过氧化氢进行。用这些氧化剂处理膜导致脂质荧光探针1,6-二苯基-1,3,5-己三烯（DPH）、3-p-(6-苯基)-1,3,5-己三烯苯丙酸（PA-DPH）以及荧光硫醇试剂N-(1-芘基)马来酰亚胺（1-PM）极化转变温度升高。过氧化作用导致5'-核苷酸酶活性降低。我们的数据支持脂质结构域膜微粘度的增加调节5'-核苷酸酶的活性。
