Oosterlynck D J, Meuleman C, Waer M, Vandeputte M, Koninckx P R
Rega Institute for Medical Research, University of Leuven, Belgium.
Fertil Steril. 1992 Aug;58(2):290-5. doi: 10.1016/s0015-0282(16)55224-8.
To investigate the local natural killer (NK) activity of the peritoneal fluid mononuclear cells (PFMC). DESIGN PATIENTS: In a prospective way, the NK activity (K562-assay) was measured in peripheral blood (PB) and peritoneal fluid (PF) of 44 women who underwent a laparoscopy for infertility and/or pain at the University Hospital of Gasthuisberg, Leuven, Belgium.
The NK activity of peripheral blood mononuclear cells and PFMC, the number and concentration of PFMC, the percentage of lymphocytes versus macrophages by May-Grünwald-Giemsa staining and the estradiol and progesterone concentration of the PF were correlated together and with the severity of endometriosis.
We demonstrated that there is a significant NK activity in PF and that this activity is decreased in women with endometriosis. This defect was more pronounced in the follicular phase of the cycle compared with the postovulatory phase. In PB of the same 44 women, the decreased NK activity correlated with the severity of the disease. This confirms our previous report on another 34 women.
The NK activity is decreased in women with endometriosis and correlated significantly with the severity of the disease in both the PB and PF of women with endometriosis.
研究腹液单核细胞（PFMC）的局部自然杀伤（NK）活性。
前瞻性地对44名因不孕和/或疼痛在比利时鲁汶加斯豪斯贝格大学医院接受腹腔镜检查的女性的外周血（PB）和腹液（PF）中的NK活性（采用K562检测法）进行测量。
外周血单核细胞和PFMC的NK活性、PFMC的数量和浓度、经May-Grünwald-Giemsa染色的淋巴细胞与巨噬细胞百分比以及PF中的雌二醇和孕酮浓度，将这些指标相互关联，并与子宫内膜异位症的严重程度相关联。
我们证明PF中存在显著的NK活性，且子宫内膜异位症女性的这种活性降低。与排卵后期相比，该缺陷在月经周期的卵泡期更为明显。在同一44名女性的PB中，NK活性降低与疾病严重程度相关。这证实了我们之前对另外34名女性的报告。
子宫内膜异位症女性的NK活性降低，且在子宫内膜异位症女性的PB和PF中均与疾病严重程度显著相关。
