Department of Physical Therapy, Program in Physical and Occupational Therapy, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
Brain Res. 2010 Oct 8;1355:165-73. doi: 10.1016/j.brainres.2010.07.070. Epub 2010 Jul 24.
It is well known that early rehabilitation is effective for functional recovery after intracerebral hemorrhage (ICH); however, the mechanisms have not been well described. The purpose of this study was to elucidate the effects of early rehabilitative therapy (treadmill running) on recovery of motor function and alteration of brain histology after ICH. Male Wistar rats, under deep anesthesia, were placed in a stereotaxic apparatus and injected with collagenase into the left striatum to induce ICH. Sham operated animals were treated with saline. All animals were randomly assigned to treadmill exercise (for 30 min/day, 9 m/min, between 4 and 14 days after surgery) and control and were designated to one of four groups: sham+control (SC), sham+treadmill (ST), ICH+control (IC), ICH+treadmill (IT). Motor deficit score (MDS) was assessed daily after surgery. Volume of tissue lost, dendritic morphology and PSD-95 protein level in the striatum were analyzed at 15 days after surgery. The MDS of IT was significantly improved compared with IC over time. There were no differences between IT and IC in the volume of tissue lost (IT: 63.8%, IC: 61.8%), spine density or PSD-95 protein level in the striatum. However, dendritic length was increased and arborization was more complex in the contralateral striatum of the IT than the IC group (IT: 1226 μm, IC: 937 μm). These data suggest that treadmill running improves motor function after ICH and that improvement may be related to alteration of dendritic morphology in the striatum.
众所周知，早期康复对于脑出血（ICH）后功能恢复是有效的；然而，其机制尚未得到很好的描述。本研究的目的是阐明早期康复治疗（跑步机运动）对脑出血后运动功能恢复和脑组织学改变的影响。雄性 Wistar 大鼠在深度麻醉下置于立体定向仪中，于左侧纹状体注射胶原酶诱导 ICH。假手术组给予生理盐水处理。所有动物随机分为跑步机运动（术后 4 至 14 天，每天 30 分钟，速度为 9 m/min）和对照组，并分为四组：假手术+对照组（SC）、假手术+跑步机运动（ST）、ICH+对照组（IC）、ICH+跑步机运动（IT）。术后每天评估运动缺陷评分（MDS）。术后 15 天分析纹状体组织丢失量、树突形态和 PSD-95 蛋白水平。与 IC 相比，IT 组的 MDS 随时间推移显著改善。IT 和 IC 之间纹状体组织丢失量（IT：63.8%，IC：61.8%）、棘密度或 PSD-95 蛋白水平无差异。然而，与 IC 组相比，IT 组对侧纹状体的树突长度增加，分支更复杂（IT：1226 μm，IC：937 μm）。这些数据表明，跑步机运动可改善脑出血后的运动功能，改善可能与纹状体树突形态改变有关。
