Department of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Antiviral Res. 2010 Oct;88(1):64-71. doi: 10.1016/j.antiviral.2010.07.006. Epub 2010 Jul 24.
Influenza viruses are important human pathogens, associated throughout history with worldwide outbreaks and pandemics. The antiviral effects of interferon (IFN)-αs/β against influenza virus infections are well recognized, yet the mechanisms whereby IFNs exert their immunomodulatory effects on an anti-influenza response remain ill-defined. Here, we describe the effects of IFN-β treatment on the immune response during a respiratory influenza (A/WSN/33) A virus infection of mice. A single dose of IFN-β (1×10(5)U) enhanced DC migration into the draining lymph node (DLN) on day 3 post-intranasal infection, and subsequently inhibited the migration from the lungs into the DLN of a newly identified late activator antigen-presenting cell population associated with type 2 immunity, LAPC. IFN-β treatment polarized the immune response towards a type 1 immune response, eliciting enhanced T(H)1 effector and cytolytic T cell responses, but diminished T(H)2 effector T cell responses in both the DLN and lung tissues of influenza virus-infected mice. Associated with the polarization towards a type 1 immune response, IFN-β treatment of mice resulted in accelerated viral clearance and diminished pulmonary eosinophilia in infected lung tissues.
流感病毒是重要的人类病原体，与历史上的全球爆发和大流行有关。干扰素（IFN）-α/β对流感病毒感染的抗病毒作用已得到广泛认可，但 IFN 发挥其免疫调节作用对抗流感反应的机制仍不清楚。在这里，我们描述了 IFN-β 处理对呼吸道流感（A/WSN/33）A 病毒感染小鼠免疫反应的影响。单次 IFN-β（1×10(5)U）处理可增强病毒感染后第 3 天进入引流淋巴结（DLN）的 DC 迁移，随后抑制与 2 型免疫相关的新鉴定的晚期激活抗原呈递细胞群（LAPC）从肺部进入 DLN 的迁移。IFN-β 处理将免疫反应向 1 型免疫反应极化，引发增强的 T(H)1 效应和细胞毒性 T 细胞反应，但在流感病毒感染小鼠的 DLN 和肺部组织中减弱 T(H)2 效应 T 细胞反应。与向 1 型免疫反应的极化相关，IFN-β 处理可加速病毒清除并减少感染肺部组织中的嗜酸性粒细胞。
