用于组织工程应用的京尼平交联聚己内酯（PCL）/明胶纳米纤维的研制。

The development of genipin-crosslinked poly(caprolactone) (PCL)/gelatin nanofibers for tissue engineering applications.

机构信息

Department of Biomedical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.

出版信息

Macromol Biosci. 2010 Jan 11;10(1):91-100. doi: 10.1002/mabi.200900168.

DOI:10.1002/mabi.200900168

PMID:19685497

Abstract

Composite nanofibers of poly(caprolactone) (PCL) and gelatin crosslinked with genipin are prepared. The contact angles and mechanical properties of crosslinked PCL-gelatin nanofibers decrease as the gelatin content increases. The proliferation of myoblasts is higher in the crosslinked PCL-gelatin nanofibers than in the PCL nanofibers, and the formation of myotubes is only observed on the crosslinked PCL-gelatin nanofibers. The expression level of myogenin, myosin heavy chain, and troponin T genes is increased as the gelatin content is increased. The results suggest that PCL-gelatin nanofibers crosslinked with genipin can be used as a substrate to modulate proliferation and differentiation of myoblasts, presenting potential applications in muscle tissue engineering.

摘要

聚己内酯（PCL）和明胶的复合纳米纤维用京尼平交联。随着明胶含量的增加，交联的 PCL-明胶纳米纤维的接触角和机械性能下降。与 PCL 纳米纤维相比，成肌细胞在交联的 PCL-明胶纳米纤维中的增殖率更高，仅在交联的 PCL-明胶纳米纤维上观察到肌管的形成。随着明胶含量的增加，成肌细胞基因的肌生成素、肌球蛋白重链和肌钙蛋白 T 的表达水平增加。结果表明，用京尼平交联的 PCL-明胶纳米纤维可用作调节成肌细胞增殖和分化的基质，在肌肉组织工程中有潜在的应用。